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Literature summary extracted from

  • Ito, S.; Taguchi, H.; Hamada, S.; Kawauchi, S.; Ito, H.; Senoura, T.; Watanabe, J.; Nishimukai, M.; Ito, S.; Matsui, H.
    Enzymatic properties of cellobiose 2-epimerase from Ruminococcus albus and the synthesis of rare oligosaccharides by the enzyme (2008), Appl. Microbiol. Biotechnol., 79, 433-441.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.3.11 overexpression in Escherichia coli Ruminococcus albus

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.3.11 4-chloromercuribenzoate
-
 Ruminococcus albus
5.1.3.11 Ag+
-
 Ruminococcus albus
5.1.3.11 Al3+
-
 Ruminococcus albus
5.1.3.11 Co2+
-
 Ruminococcus albus
5.1.3.11 Cu2+
-
 Ruminococcus albus
5.1.3.11 Fe3+
-
 Ruminococcus albus
5.1.3.11 iodoacetate
-
 Ruminococcus albus
5.1.3.11 N-bromosuccinimide
-
 Ruminococcus albus
5.1.3.11 Pb2+
-
 Ruminococcus albus
5.1.3.11 Zn2+
-
 Ruminococcus albus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.3.11 13.8
-
 cellobiose pH 7.5, 30°C Ruminococcus albus
5.1.3.11 33
-
 lactose pH 7.5, 30°C Ruminococcus albus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.3.11 43100
-
 1 * 43100, SDS-PAGE Ruminococcus albus
5.1.3.11 44000
-
 gel filtration Ruminococcus albus

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.11 Ruminococcus albus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.3.11 recombinant enzyme Ruminococcus albus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.1.3.11 28.4
-
 substrate lactose, pH 7.5, 30°C Ruminococcus albus
5.1.3.11 51
-
 substrate cellobiose, pH 7.5, 30°C Ruminococcus albus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.11 cellobiose
-
 Ruminococcus albus 4-O-beta-D-glucopyranosyl-D-mannose
-
 ?
5.1.3.11 lactose
-
 Ruminococcus albus 4-O-beta-D-galactopyranosyl-D-mannose i.e. epilactose ?
5.1.3.11 additional information no substrates: N-acetyl-D-glucosamine, uridine 5'-diphosphate-glucose, D-glucose 6-phosphate, maltose, sophorose, laminaribiose, and gentiobiose Ruminococcus albus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.1.3.11 monomer 1 * 43100, SDS-PAGE Ruminococcus albus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.1.3.11 30
-
-
 Ruminococcus albus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.1.3.11 52.1
-
 lactose pH 7.5, 30°C Ruminococcus albus
5.1.3.11 63.8
-
 cellobiose pH 7.5, 30°C Ruminococcus albus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.3.11 7.5
-
-
 Ruminococcus albus