Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zhang W.W.
    Sun, K.; Cheng, S.; Sun, L.: Characterization of DegQVh, a serine protease and a protective immunogen from a pathogenic Vibrio harveyi strain (2008), Appl. Environ. Microbiol., 74, 6254-6262.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.4.21.B50 medicine the purified recombinant DegQVh is a protective immunogen that could confer protection upon fish against infection by Vibrio harveyi. In order to improve the efficiency of DegQVh as a vaccine, a genetic construct in the form of the plasmid pAQ1 is built, in which the DNA encoding the processed DegQVh protein is fused with the DNA encoding the secretion region of AgaV, an extracellular beta-agarase. The Escherichia coli strain harboring pAQ1 can express and secrete the chimeric DegQVh protein into the culture supernatant. Vaccination of fish with viable Escherichia coli expressing chimeric degQVh significantly enhanced the survival of fish against Vibrio harveyi challenge, which is possibly due to the relatively prolonged exposure of the immune system to the recombinant antigen produced constitutively Vibrio harveyi

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.B50 expression in Escherichia coli Vibrio harveyi

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.B50 D113A mutant exhibits 97.5% reduction in activity Vibrio harveyi
3.4.21.B50 H83A mutant exhibits 93.4% reduction in activity Vibrio harveyi
3.4.21.B50 S188A mutant exhibits 91.8% reduction in activity Vibrio harveyi

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.B50 phenylmethanesulfonyl fluoride
-
 Vibrio harveyi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.B50 Mn2+ 10 mM, 42% increase in activity Vibrio harveyi

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.B50 Vibrio harveyi B6CND6 strain T4, a pathogenic strain isolated from diseased fish
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.B50 recombinant enzyme Vibrio harveyi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.B50 casein + H2O endoprotease, active serine protease whose activity requires the integrity of the catalytic site and the PDZ domains Vibrio harveyi hydrolyzed casein
-
 ?
3.4.21.B50 additional information recombinant DegQVh can not degrade bovine serum albumin and collagen. The purified recombinant DegQVh is a protective immunogen that could confer protection upon fish against infection by Vibrio harveyi Vibrio harveyi ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.B50 DegQ
-
 Vibrio harveyi
3.4.21.B50 DegQVh
-
 Vibrio harveyi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.B50 50
-
 recombinant enzyme Vibrio harveyi

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.21.B50 40 55 exhibits more than 70% of the maximum activity over the temperature range of 40 to 55°C Vibrio harveyi

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.B50 50
-
 1 h, enzyme retains 81% activity Vibrio harveyi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.B50 8
-
 recombinant enzyme Vibrio harveyi

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.21.B50 5 9 exhibits more than 70% of the maximum activity over the pH range of 5 to 9 Vibrio harveyi