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Literature summary extracted from

  • Nilgiriwala, K.S.; Alahari, A.; Rao, A.S.; Apte, S.K.
    Cloning and overexpression of alkaline phosphatase PhoK from Sphingomonas sp. strain BSAR-1 for bioprecipitation of uranium from alkaline solutions (2008), Appl. Environ. Microbiol., 74, 5516-5523.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.1.3.1 biotechnology the data demonstrate the potential utility of genetically engineering PhoK for the bioprecipitation of uranium from alkaline solutions Sphingomonas sp. BSAR-1

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.1 phoK is cloned and overexpressed in Escherichia coli strain BL21(DE3). The resultant Escherichia coli strain EK4 overexpress cellular activity 55times higher and secrete extracellular PhoK activity 13times higher than Sphingomonas sp. strain BSAR-1. The recombinant strain very rapidly precipitate more that 90% of input uranium in less than 2 h from alkaline solutions (pH 9) containing 0.5 to 5 mM of uranyl carbonate. In recombinant strain EK4, precipitated uranium remains cell bound. The EK4 cells exhibited a much higher loading capacity of 3.8 g uranium/g dry weight in less than 2 h compared to only 1.5 g uranium/g dry weight in more than 7 h in strain BSAR-1 Sphingomonas sp. BSAR-1

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.1 60000
-
 x * 60000, SDS-PAGE Sphingomonas sp. BSAR-1
3.1.3.1 200000
-
 approximately, gel filtration Sphingomonas sp. BSAR-1

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.1 Sphingomonas sp. BSAR-1 A1YYW7
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.1 4-nitrophenyl phosphate + H2O
-
 Sphingomonas sp. BSAR-1 4-nitrophenol + phosphate
-
 ?
3.1.3.1 beta-glycerophosphate + H2O uranium precipitation assay: uranyl carbonate solution in carbonate-bicarbonate buffer, supplemented with beta-glycerophosphate as the substrate at 30°C under static nongrowing conditions in a final volume of 5 ml Sphingomonas sp. BSAR-1 glycerol + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.1 multimer x * 60000, SDS-PAGE Sphingomonas sp. BSAR-1

Synonyms

EC Number Synonyms Comment Organism
3.1.3.1 alkaline phosphatase
-
 Sphingomonas sp. BSAR-1
3.1.3.1 phoK
-
 Sphingomonas sp. BSAR-1

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.1 30
-
 assay at Sphingomonas sp. BSAR-1

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.1 9
-
 assay at Sphingomonas sp. BSAR-1