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Literature summary extracted from

  • Meijnen, J.P.; de Winde, J.H.; Ruijssenaars, H.J.
    Engineering Pseudomonas putida S12 for efficient utilization of D-xylose and L-arabinose (2008), Appl. Environ. Microbiol., 74, 5031-5037.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.7.1.17 biotechnology putative use of lignocellulosic biomass as feedstock for the chemical industry Escherichia coli
5.3.1.5 biotechnology putative use of lignocellulosic biomass as feedstock for the chemical industry Escherichia coli
5.3.1.5 synthesis expression of Escherichia coli xylose isomerase and xylulokinase in Pseudomonas putida S12 for efficient utilization of D-xylose and L-arabinose. After laboratory evolution of strains by repeated transfer to fresh minimal medium with xylose, a strain that efficiently utilizes xylose at a considerably improved growth rate can be obtained. The high yield can be attributed in part to glucose dehydrogenase inactivity, whereas the improved growth rate may be connected to alterations in the primary metabolism. The evolved D-xylose-utilizing strain metabolizes L-arabinose as efficiently as D-xylose, while its ability to utilize glucose is not affected Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.17 cloning and functional expression of enzymes from Escherichia coli in Pseudomonas putida Escherichia coli
5.3.1.5 cloning and functional expression of enzymes from Escherichia coli in Pseudomonas putida Escherichia coli
5.3.1.5 expression in Pseudomonas putida S12 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.17 Mg2+ included in assay medium Escherichia coli
5.3.1.5 Mg2+ included in assay medium Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.17 D-xylulose + ATP Escherichia coli
-
 D-xylulose 5-phosphate + ADP
-
 ?
2.7.1.17 L-ribulose + ATP Escherichia coli
-
 L-ribulose 5-phosphate + ADP
-
 ?
5.3.1.5 D-Xylose Escherichia coli
-
 D-Xylulose
-
 ?
5.3.1.5 L-arabinose Escherichia coli
-
 L-ribulose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.17 Escherichia coli
-
-
-
5.3.1.5 Escherichia coli
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.17 cell culture Pseudomonas strain S12 containing xylose isomerase gene and xylulokinase gene from Escherichia coli strain DH5 alpha Escherichia coli
-
5.3.1.5 cell culture Pseudomonas strain S12 containing xylose isomerase gene and xylulokinase gene from Escherichia coli strain DH5 alpha Escherichia coli
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.17 0.134
-
 Pseudomonas putida S12 strain xylAB containing xylose isomerase and xylulokinase gene from Escherichia coli Escherichia coli
5.3.1.5 0.034
-
 Pseudomonas putida S12 strain xylAB containing xylose isomerase and xylulokinase gene from Escherichia coli Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.17 D-xylulose + ATP
-
 Escherichia coli D-xylulose 5-phosphate + ADP
-
 ?
2.7.1.17 L-ribulose + ATP
-
 Escherichia coli L-ribulose 5-phosphate + ADP
-
 ?
5.3.1.5 D-Xylose
-
 Escherichia coli D-Xylulose
-
 ?
5.3.1.5 L-arabinose
-
 Escherichia coli L-ribulose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.17 xylulokinase
-
 Escherichia coli
5.3.1.5 xylose isomerase
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.17 30
-
 assay at Escherichia coli
5.3.1.5 30
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.17 7.5
-
 assay at Escherichia coli
5.3.1.5 7.5
-
 assay at Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.17 ATP
-
 Escherichia coli