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Literature summary extracted from

  • Iwamoto, S.; Motomura, K.; Shinoda, Y.; Urata, M.; Kato, J.; Takiguchi, N.; Ohtake, H.; Hirota, R.; Kuroda, A.
    Use of an Escherichia coli recombinant producing thermostable polyphosphate kinase as an ATP regenerator to produce fructose 1,6-diphosphate (2007), Appl. Environ. Microbiol., 73, 5676-5678.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.7.4.1 synthesis the enzyme is useful for ATP production from polyphosphate Thermus thermophilus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.1 expression in Escherichia coli Thermus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.1 Mg2+
-
 Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.1 ATP + (phosphate)n Thermus thermophilus
-
 ADP + (phosphate)n+1
-
 r
2.7.4.1 ATP + (phosphate)n Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
-
 ADP + (phosphate)n+1
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.1 Thermus thermophilus Q72JY1
-
-
2.7.4.1 Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 Q72JY1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.1 ATP + (phosphate)n
-
 Thermus thermophilus ADP + (phosphate)n+1
-
 r
2.7.4.1 ATP + (phosphate)n
-
 Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 ADP + (phosphate)n+1
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.7.4.1 polyP kinase
-
 Thermus thermophilus
2.7.4.1 PPK
-
 Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.4.1 70
-
 assay at Thermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.4.1 70
-
 recombinant enzyme in Escherichia coli cells, 10 min, then mixed with 50 mM MOPS, pH 6.0, containing 0.08 mM ADP, 40 mM (NH4)2SO4, 4 mM MgCl2, and 5 mM polyP65, the activity is detected in the precipitate. After one week, 60% of the activity is still remaining Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.1 7.2
-
 assay at Thermus thermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.4.1 ATP
-
 Thermus thermophilus