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Literature summary extracted from
- Biochemical characterization of raw-starch-digesting alpha amylase purified from Bacillus amyloliquefaciens (2009), Appl. Biochem. Biotechnol., 158, 653-662.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.1 | EDTA | 80% of the enzyme activity is lost when the enzyme is incubated with 10 mM EDTA, indicating the enzyme is a metalloenzyme, pH 5.0, 50°C | Bacillus amyloliquefaciens |  |
| 3.2.1.1 | K+ | inhibits enzyme activity, 60 and 42% relative activity with 5 and 10 mM K+, pH 5.0, 50°C | Bacillus amyloliquefaciens | |
| 3.2.1.1 | Mg2+ | shows no significant effect with 5 mM Mg2+, 92% relative activity, lead to inhibition with 10 mM, 48% relative activity, pH 5.0, 50°C | Bacillus amyloliquefaciens | |
| 3.2.1.1 | Mn2+ | inhibits enzyme activity, 50 and 21% relative activity with 5 and 10 mM Mn2+, pH 5.0, 50°C | Bacillus amyloliquefaciens | |
| 3.2.1.1 | SDS | retains 30 and 12% relative activity after inhibition with 5 and 10 mM SDS, respectively, pH 5.0, 50°C | Bacillus amyloliquefaciens | |
| 3.2.1.1 | Urea | retains 58 and 16% relative activity after inhibition with 5 and 10 mM urea, respectively, pH 5.0, 50°C | Bacillus amyloliquefaciens | |
| 3.2.1.1 | Zn2+ | shows no significant effect with 5 mM Zn2+, 90% relative activity, lead to inhibition with 10 mM, 56% relative activity | Bacillus amyloliquefaciens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.1 | additional information | - |
amylose | Km: 2.34 mg and Vmax: 3.047 mg/min | Bacillus amyloliquefaciens | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.1 | Ca2+ | enhances the enzyme activity with 5 mM Ca2+, 120% relative activity, and with 10 mM 96% relative activity, pH 5.0, 50°C | Bacillus amyloliquefaciens | |
| 3.2.1.1 | Cu2+ | enhances the enzyme activity with 5 mM Cu2+, 120% relative activity, and with 10 mM 87% relative activity, pH 5.0, 50°C | Bacillus amyloliquefaciens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 58000 | - |
SDS-PAGE | Bacillus amyloliquefaciens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.1 | Bacillus amyloliquefaciens | - |
ATCC 23842 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.1 | 38.46fold purified, to near homogeneity | Bacillus amyloliquefaciens |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 72 | - |
purified to near homogeneity, pH 5.0, 50°C | Bacillus amyloliquefaciens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.1 | amylose + H2O | 120% relative enzyme activity compared to reaction with soluble starch as substrate, pH 5.0, 50°C | Bacillus amyloliquefaciens | ? | - |
? | |
| 3.2.1.1 | corn starch + H2O | 62% relative enzyme activity compared to reaction with soluble starch as substrate, pH 5.0, 50°C | Bacillus amyloliquefaciens | ? | - |
? | |
| 3.2.1.1 | potato starch + H2O | 95% relative enzyme activity compared to reaction with soluble starch as substrate, pH 5.0, 50°C | Bacillus amyloliquefaciens | ? | - |
? | |
| 3.2.1.1 | rice starch + H2O | 43% relative enzyme activity compared to reaction with soluble starch as substrate, pH 5.0, 50°C | Bacillus amyloliquefaciens | ? | - |
? | |
| 3.2.1.1 | starch + H2O | soluble starch as substrate, pH 5.0, 50°C, 100% relative enzyme activity | Bacillus amyloliquefaciens | ? | the liberated reducing sugars (glucose equivalents) are estimated by the dinitrosalicylic acid method | ? | |
| 3.2.1.1 | wheat starch + H2O | 56.5% relative enzyme activity compared to reaction with soluble starch as substrate, pH 5.0, 50°C | Bacillus amyloliquefaciens | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.1 | ? | x * 58000, SDS-PAGE | Bacillus amyloliquefaciens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.1 | alpha amylase | - |
Bacillus amyloliquefaciens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 50 | - |
optimum and assay at | Bacillus amyloliquefaciens |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 40 | 80 | 40, 50, 60, 70, and 80°C with relative activities of 78, 100, 80, 40, and 18%, respectively | Bacillus amyloliquefaciens |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 60 | - |
in the absence of CaCl2, the enzyme is stable up to 25 min retaining 65% of the activity. In the presence of 5 mM CaCl2 90% of the activity retained after 25 min incubation at 60°C | Bacillus amyloliquefaciens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 5 | - |
optimum and assay at | Bacillus amyloliquefaciens |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 4 | 10 | pH 4.0, 5.0, 6.0, 7.0, 8.0, 9.0, and 10.0 with relative activities of 62, 100, 82, 78, 65, 35, and 20%, respectively | Bacillus amyloliquefaciens |
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