EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.4 | alpha-chymotrypsin | denaturant | Aspergillus oryzae | |
3.2.1.4 | Urea | denaturant | Aspergillus oryzae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.4 | additional information | - |
additional information | KM value: 20 mg carboxymethylcellulose/ml. Vmax value: 854 U/mg protein, one unit defined as the release of 1 mircomol of glucose equivalent per minute from carboxymethylcellulose | Aspergillus oryzae |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.2.1.4 | extracellular | - |
Aspergillus oryzae | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.4 | 25000 | - |
SDS-PAGE | Aspergillus oryzae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.4 | Aspergillus oryzae | - |
cmc-1 | - |
3.2.1.4 | Aspergillus oryzae cmc-1 | - |
cmc-1 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.4 | ammonium sulfate precipitation and various column chromatography on fast protein liquid chromatography system to homogeneity | Aspergillus oryzae |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.4 | 92.5 | - |
gel filtration chromatography, last purification step | Aspergillus oryzae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.4 | carboxymethylcellulose + H2O | - |
Aspergillus oryzae | ? | - |
? | |
3.2.1.4 | carboxymethylcellulose + H2O | - |
Aspergillus oryzae cmc-1 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.4 | monomer | SDS-PAGE | Aspergillus oryzae |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.4 | CMCase | - |
Aspergillus oryzae |
3.2.1.4 | endoglucanase | - |
Aspergillus oryzae |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.4 | 55 | - |
- |
Aspergillus oryzae |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.4 | 47 | - |
enzyme is stable up to 47°C and exhibits half-life of 138.6 min | Aspergillus oryzae |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.4 | additional information | - |
additional information | 356 1/s, one unit defined as the release of 1 mircomol of glucose equivalent per minute from carboxymethylcellulose | Aspergillus oryzae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.4 | 4.4 | - |
- |
Aspergillus oryzae |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.4 | 4.1 | 5.3 | enzyme is stable over a pH range of 4.1-5.3 | Aspergillus oryzae |