Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Javed, M.R.; Rashid, M.H.; Nadeem, H.; Riaz, M.; Perveen, R.
    Catalytic and Thermodynamic Characterization of Endoglucanase (CMCase) from Aspergillus oryzae cmc-1 (2008), Appl. Biochem. Biotechnol., 157, 483-497.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.4 alpha-chymotrypsin denaturant Aspergillus oryzae
3.2.1.4 Urea denaturant Aspergillus oryzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.4 additional information
-
additional information KM value: 20 mg carboxymethylcellulose/ml. Vmax value: 854 U/mg protein, one unit defined as the release of 1 mircomol of glucose equivalent per minute from carboxymethylcellulose Aspergillus oryzae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.4 extracellular
-
Aspergillus oryzae
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.4 25000
-
SDS-PAGE Aspergillus oryzae

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Aspergillus oryzae
-
cmc-1
-
3.2.1.4 Aspergillus oryzae cmc-1
-
cmc-1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4 ammonium sulfate precipitation and various column chromatography on fast protein liquid chromatography system to homogeneity Aspergillus oryzae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.4 92.5
-
gel filtration chromatography, last purification step Aspergillus oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 carboxymethylcellulose + H2O
-
Aspergillus oryzae ?
-
?
3.2.1.4 carboxymethylcellulose + H2O
-
Aspergillus oryzae cmc-1 ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 monomer SDS-PAGE Aspergillus oryzae

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 CMCase
-
Aspergillus oryzae
3.2.1.4 endoglucanase
-
Aspergillus oryzae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 55
-
-
Aspergillus oryzae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 47
-
enzyme is stable up to 47°C and exhibits half-life of 138.6 min Aspergillus oryzae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.4 additional information
-
additional information 356 1/s, one unit defined as the release of 1 mircomol of glucose equivalent per minute from carboxymethylcellulose Aspergillus oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 4.4
-
-
Aspergillus oryzae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.4 4.1 5.3 enzyme is stable over a pH range of 4.1-5.3 Aspergillus oryzae