EC Number | Cloned (Comment) | Organism |
---|---|---|
1.5.1.3 | gene dhfr-ts, DNA and amino acid sequence determination and analysis, a 1,548-bp open reading frame encoding a 58.8-kDa translated peptide containing DHFR and TS domains linked together in a single polypeptide chain, expression of the GST-tagged enzyme in Escherichia coli strain BL21(DE3) as soluble protein, expression as GFP-tagged enzyme in Babesia gibsoni cytoplasm | Babesia gibsoni |
2.1.1.45 | expression in Escherichia coli | Babesia gibsoni |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.3 | methotrexate | - |
Babesia gibsoni | |
1.5.1.3 | pyrimethamine | - |
Babesia gibsoni | |
1.5.1.3 | trimethoprim | - |
Babesia gibsoni | |
2.1.1.45 | antifolate | inhibits activitiy of bifunctional DHFR-TS enzyme | Babesia gibsoni |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.3 | 0.0016 | - |
7,8-dihydrofolate | 25°C, recombinant GST-tagged enzyme | Babesia gibsoni | |
1.5.1.3 | 0.0047 | - |
7,8-dihydrofolate | 25°C, recombinant detagged enzyme | Babesia gibsoni | |
1.5.1.3 | 0.00975 | - |
NADPH | 25°C, recombinant detagged enzyme | Babesia gibsoni | |
1.5.1.3 | 0.017 | - |
NADPH | 25°C, recombinant GST-tagged enzyme | Babesia gibsoni |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.5.1.3 | cytoplasm | - |
Babesia gibsoni | 5737 | - |
2.1.1.45 | cytoplasm | - |
Babesia gibsoni | 5737 | - |
2.1.1.45 | soluble | - |
Babesia gibsoni | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.5.1.3 | 57000 | - |
x * 57000, recombinant detagged enzyme, SDS-PAGE | Babesia gibsoni |
2.1.1.45 | 58000 | - |
SDS-PAGE, bifunctional native BgDHFR-TS enzyme from the parasite lysate | Babesia gibsoni |
2.1.1.45 | 58800 | - |
x * 58800, calculated, 57000, SDS-PAGE | Babesia gibsoni |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.3 | 7,8-dihydrofolate + NADPH + H+ | Babesia gibsoni | - |
5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + deoxyuridine monophosphate | Babesia gibsoni | - |
7,8-dihydrofolate + deoxythymidine monophosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.3 | Babesia gibsoni | B5U9U8 | gene dhfr-ts | - |
2.1.1.45 | Babesia gibsoni | - |
bifunctional dihydrofolate reductase-thymidylate synthase | - |
2.1.1.45 | Babesia gibsoni | B5U9U8 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.1.3 | recombinant GST-tagged enzyme from Escherichia coli by glutathione affinity chromatography, the tag is cleaved off | Babesia gibsoni |
2.1.1.45 | glutathione elusion method | Babesia gibsoni |
2.1.1.45 | recombinant protein | Babesia gibsoni |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.1.1.45 | merozoite | - |
Babesia gibsoni | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.3 | 7,8-dihydrofolate + NADPH + H+ | - |
Babesia gibsoni | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
1.5.1.3 | additional information | a bifunctional enzyme with dihydrofolate reductase and thymidylate synthase activities, overview | Babesia gibsoni | ? | - |
? | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + deoxyuridine monophosphate | - |
Babesia gibsoni | 7,8-dihydrofolate + deoxythymidine monophosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.5.1.3 | ? | x * 57000, recombinant detagged enzyme, SDS-PAGE | Babesia gibsoni |
2.1.1.45 | ? | x * 58800, calculated, 57000, SDS-PAGE | Babesia gibsoni |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.5.1.3 | DHFR-TS | - |
Babesia gibsoni |
1.5.1.3 | dihydrofolate reductase-thymidylate synthase | - |
Babesia gibsoni |
2.1.1.45 | BgDHFR-TS | - |
Babesia gibsoni |
2.1.1.45 | DHFR-TS | - |
Babesia gibsoni |
2.1.1.45 | dihydrofolate reductase-thymidylate synthase | - |
Babesia gibsoni |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.5.1.3 | 25 | - |
assay at | Babesia gibsoni |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.3 | NADPH | - |
Babesia gibsoni |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.3 | additional information | - |
additional information | antifolate inhibition kinetics, overview | Babesia gibsoni |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
1.5.1.3 | 0.0000686 | - |
25°C, recombinant detagged enzyme | Babesia gibsoni | methotrexate | |
1.5.1.3 | 0.05 | - |
25°C, recombinant detagged enzyme | Babesia gibsoni | trimethoprim | |
1.5.1.3 | 0.055 | - |
25°C, recombinant detagged enzyme | Babesia gibsoni | pyrimethamine |