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Literature summary extracted from

  • Keller, S.; Wage, T.; Hohaus, K.; Hölzer, M.; Eichhorn, E.; van Pee, K.H.
    Purification and Partial Characterization of Tryptophan 7-Halogenase (PrnA) from Pseudomonas fluorescens (2000), Angew. Chem. Int. Ed. Engl., 39, 2300-2302.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.9 L-tryptophan + FADH2 + Cl- + O2 + H+ Pseudomonas fluorescens the enzyme is involved in biosynthesis of pyrrolnitrin 7-chloro-L-tryptophan + FAD + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.9 Pseudomonas fluorescens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.19.9
-
 Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.19.9 additional information
-
 PrnA activities are determined after addition of flavin reductase (SsuE). Without reductase, fractions after elution from the molecular sieve column do not show any activity Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.9 L-tryptophan + FADH2 + Cl- + O2 + H+ the enzyme is involved in biosynthesis of pyrrolnitrin Pseudomonas fluorescens 7-chloro-L-tryptophan + FAD + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.19.9 PrnA
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 Pseudomonas fluorescens
1.14.19.9 Trp-7-Hal
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 Pseudomonas fluorescens

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.9 FADH2 the enzyme is specific for FADH2 Pseudomonas fluorescens