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Literature summary extracted from

  • Kato, N.; Miyamoto, N.; Shimao, M.; Sakazawa, C.
    3-Hexulose phosphate synthase from a new facultative methylotroph, Mycobacterium gastri MB19 (1988), Agric. Biol. Chem., 52, 2659-2661.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.43 glycolic acid glycolic acid inhibits the enzyme competitively with respect to formaldehyde Mycobacterium gastri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.43 1.4
-
 formaldehyde apparent value, in 50 mM potassium phosphate, pH 7.5, with 5 mM MgCl2, at 30°C Mycobacterium gastri
4.1.2.43 4.3
-
 glycolaldehyde apparent value, in 50 mM potassium phosphate, pH 7.5, with 5 mM MgCl2, at 30°C Mycobacterium gastri
4.1.2.43 5.7
-
 methylglyoxal apparent value, in 50 mM potassium phosphate, pH 7.5, with 5 mM MgCl2, at 30°C Mycobacterium gastri

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.2.43 Mg2+ 1 mM Mg2+ is absolutely required for the enzyme activity and stability Mycobacterium gastri
4.1.2.43 Mn2+ Mg2+ can be replaced by Mn2+ (1 mM) Mycobacterium gastri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.43 24000
-
 SDS-PAGE Mycobacterium gastri
4.1.2.43 43000
-
 native enzyme, gel filtration Mycobacterium gastri

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.43 Mycobacterium gastri
-
-
-
4.1.2.43 Mycobacterium gastri MB19
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.43 phenyl-Sepharose column chromatography and DEAE-Sephacel column chromatography Mycobacterium gastri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.43 3.2
-
 cell free extract, at 30°C Mycobacterium gastri
4.1.2.43 74.2
-
 after 23fold purification, at 30°C Mycobacterium gastri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.43 D-ribulose 5-phosphate + formaldehyde
-
 Mycobacterium gastri D-arabino-hex-3-ulose 6-phosphate
-
 ?
4.1.2.43 D-ribulose 5-phosphate + formaldehyde
-
 Mycobacterium gastri MB19 D-arabino-hex-3-ulose 6-phosphate
-
 ?
4.1.2.43 D-ribulose 5-phosphate + glycolaldehyde
-
 Mycobacterium gastri 4-heptulose 7-phosphate
-
 ?
4.1.2.43 D-ribulose 5-phosphate + glycolaldehyde
-
 Mycobacterium gastri MB19 4-heptulose 7-phosphate
-
 ?
4.1.2.43 D-ribulose 5-phosphate + methylglyoxal
-
 Mycobacterium gastri ?
-
 ?
4.1.2.43 D-ribulose 5-phosphate + methylglyoxal
-
 Mycobacterium gastri MB19 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.2.43 3-hexulose phosphate synthase
-
 Mycobacterium gastri
4.1.2.43 HPS
-
 Mycobacterium gastri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.2.43 30
-
-
 Mycobacterium gastri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.43 7.5 8
-
 Mycobacterium gastri