EC Number | Cloned (Comment) | Organism |
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4.4.1.5 | the glyoxalase-1 homologue CeGly is subcloned into a Green Fluorescent Protein (GFP) vector under control of its native promotor. Enzymatic activity of CeGly in cultures of age-synchronized 1-day-old transgenic Caenorhabditis elegans overexpressing CeGly is ca. 200fold higher than in the wild-type strain. Increased enzymatic activity in transgenic animals results in a significant reduction of both methylglyoxal and methylglyoxal-derived arginine- and lysine-derived adducts. Increased glyoxalase-1 activity significantly prolongs lifespan. Mean lifespan increases in transgenic animals from 13.3 days to 17.2 days and maximum lifespan from 28 days to 37 days | Caenorhabditis elegans |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.4.1.5 | H2O2 | 2 mM, 2 h, significantly reduces enzyme activity from 0.2 U/single Caenorhabditis elegans (without addition of H2O2) to 0.065 U/single Caenorhabditis elegans | Caenorhabditis elegans | |
4.4.1.5 | methylglyoxal | 200 mM, 8 h, significantly reduces enzyme activity from 0.2 U/single Caenorhabditis elegans (without addition of methylglyoxal) to 0.12 U/single Caenorhabditis elegans | Caenorhabditis elegans |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
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EC Number | Organism | UniProt | Comment | Textmining |
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4.4.1.5 | Caenorhabditis elegans | - |
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EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
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4.4.1.5 | additional information | - |
enzyme activity decreases significantly from 0.2 U/single Caenorhabditis elegans at day 1 to 0.02 U/single Caenorhabditis elegans at day 12. In contrast mRNA expression of the homologue, CeGly did not decrease at day 12 but slightly increase, suggesting that post-transciptional modification of glyoxalase-1 protein, rather than decrease transcription, accounted for the age-related decrease in enzyme activity. The glyoxalase-1 homologue CeGly is subcloned into a Green Fluorescent Protein (GFP) vector under control of its native promotor. Enzymatic activity of CeGly in cultures of age-synchronized 1-day-old transgenic Caenorhabditis elegans overexpressing CeGly is ca. 200fold higher than in the wild-type strain. Increased enzymatic activity in transgenic animals results in a significant reduction of both methylglyoxal and methylglyoxal-derived arginine- and lysine-derived adducts. Increased glyxalase-1 activity significantly prolongs lifespan. Mean lifespan increases in transgenic animals from 13.3 days to 17.2 days and maximum lifespan from 28 days to 37 days | Caenorhabditis elegans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
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4.4.1.5 | additional information | the glyxalase-1 homologue CeGly is subcloned into a Green Fluorescent Protein (GFP) vector under control of its native promotor. Enzymatic activity of CeGly in cultures of age-synchronized 1-day-old transgenic Caenorhabditis elegans overexpressing CeGly is ca. 200fold higher than in the wild-type strain. Increased enzymatic activity in transgenic animals results in a significant reduction of both methylglyoxal and methylglyoxal-derived arginine- and lysine-derived adducts. Increased glyxalase-1 activity significantly prolongs lifespan. Mean lifespan increases in transgenic animals from 13.3 days to 17.2 days and maximum lifespan from 28 days to 37 days | Caenorhabditis elegans | ? | - |
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EC Number | Synonyms | Comment | Organism |
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4.4.1.5 | glyoxalase-1 | - |
Caenorhabditis elegans |