EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.11 | EDTA | - |
Aspergillus pseudoglaucus | |
3.1.1.11 | HgCl2 | 1.0 mM of HgCl2 results in approximately 50% loss of enzyme activity, while concentration of 8 mM produces complete loss of enzyme activity | Aspergillus pseudoglaucus | |
3.1.1.11 | iodoacetic acid | - |
Aspergillus pseudoglaucus | |
3.1.1.11 | PbCl2 | - |
Aspergillus pseudoglaucus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.11 | Ca2+ | the highest PME activity occurrs at 20 mM of Ca2+, further increase in concentration results in the decline of the enzyme activity | Aspergillus pseudoglaucus | |
3.1.1.11 | K+ | the activity of the enzyme increases with increase in the concentration of K+, further increase in concentration results in the decline of the enzyme activity | Aspergillus pseudoglaucus | |
3.1.1.11 | Mg2+ | highest activity at 15 mM Mg2+, further increase in concentration results in the decline of the enzyme activity | Aspergillus pseudoglaucus | |
3.1.1.11 | Na+ | the activity of the enzyme increases with increase in the concentration of Na+, further increase in concentration results in the decline of the enzyme activity | Aspergillus pseudoglaucus | |
3.1.1.11 | Zn2+ | highest activity at 15 mM Zn2+, further increase in concentration results in the decline of the enzyme activity | Aspergillus pseudoglaucus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.1.11 | 141300 | - |
gel filtration | Aspergillus pseudoglaucus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.11 | Aspergillus pseudoglaucus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.11 | ammonium sulfate precipitation, Sephadex G-100 gel filtration, and Sephadex C-50 gel filtration | Aspergillus pseudoglaucus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.1.11 | 0.82 | - |
crude extract | Aspergillus pseudoglaucus |
3.1.1.11 | 6.5 | - |
after 7.93fold purification | Aspergillus pseudoglaucus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.11 | pectin + H2O | - |
Aspergillus pseudoglaucus | methanol + pectate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.11 | pectin methylesterase | - |
Aspergillus pseudoglaucus |
3.1.1.11 | PME | - |
Aspergillus pseudoglaucus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.11 | 30 | - |
- |
Aspergillus pseudoglaucus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.11 | 30 | - |
the optimum temperature for the activity of pectimethylesterase is 30°C, however, within 10 min of heating approximately 50% loss of enzyme activity is lost, additional 10 min of heating results in retention of only 9% enzyme activity, and further heating results in complete loss of enzyme activity | Aspergillus pseudoglaucus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.11 | 6.5 | - |
- |
Aspergillus pseudoglaucus |