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Literature summary extracted from

  • Pendini, N.R.; Polyak, S.W.; Booker, G.W.; Wallace, J.C.; Wilce, M.C.
    Purification, crystallization and preliminary crystallographic analysis of biotin protein ligase from Staphylococcus aureus (2008), Acta Crystallogr. Sect. F, F64, 520-523.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.3.4.15 purified recombinant enzyme, hanging-drop vapour-diffusion method, 0.001 ml of 15 mg/ml protein solution is mixed with 0.001 ml of reservoir solution containing 8% PEG 8000 and 0.1 M Tris–HCl pH 8.0., at 4°C or 20°C, crystals appear within 24 h, X-ray diffraction structure determination and analysis at 2.3 A resolution Staphylococcus aureus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.4.15 Mg2+
-
 Staphylococcus aureus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.4.15 ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] Staphylococcus aureus
-
 AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.15 Staphylococcus aureus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.15 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and ultrafiltration Staphylococcus aureus

Storage Stability

EC Number Storage Stability Organism
6.3.4.15 -80°C, 1-2 mg/ml purified recombinant His6-tagged enzyme, in 50 mM Tris-HCl, pH 7.5, 0.5 mM EDTA, pH 8.0, 250 mM NaCl, at least 6 months without loss of activity Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.15 ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 Staphylococcus aureus AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.15 additional information biotin protein ligase from Staphylococcus aureus catalyses the biotinylation of acetyl-CoA carboxylase and pyruvate carboxylase Staphylococcus aureus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.4.15 biotin protein ligase
-
 Staphylococcus aureus
6.3.4.15 BPL
-
 Staphylococcus aureus

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.4.15 ATP
-
 Staphylococcus aureus