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Literature summary extracted from

  • Gupta, V.; Gupta, R.K.; Khare, G.; Surolia, A.; Salunke, D.M.; Tyagi, A.K.
    Crystallization and preliminary X-ray diffraction analysis of biotin acetyl-CoA carboxylase ligase (BirA) from Mycobacterium tuberculosis (2008), Acta Crystallogr. Sect. F, 64, 524-527.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
6.3.4.15 drug development the enzyme is a target for the design of effective antitubercular drugs Mycobacterium tuberculosis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.4.15 gene Rv3279c, DNA and amino acid sequence determination and analysis, expression of C-terminally Strep-tagged BirA in Escherichia coli Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.3.4.15 purified recombinant enzyme, 0.003 ml of 5 mg/ml protein in 2.5 mM desthiobiotin in 20 mM Tris-HCl pH 8.0, 50 mM NaCl, 1 mM PMSF and 1 mM DTT, is mixed with 0.003 ml of reservoir solution containing 12-16% w/v of both PEG 4000 and PEG 8000 in 0.1 M HEPES, pH 7.5, X-ray diffraction structure determination and analysis at 2.8 A resolution Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.4.15 Mg2+
-
 Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.4.15 ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] Mycobacterium tuberculosis
-
 AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.15 additional information Mycobacterium tuberculosis biotin-dependent acetyl-CoA carboxylases/transcarboxylases are a class of enzymes that, in addition to fatty-acid biosynthesis, are important for gluconeogenesis as well as propionate catabolism ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.15 Mycobacterium tuberculosis P96884 gene Rv3279c
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.15 recombinant C-terminally Strep-tagged BirA from Escherichia coli Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.15 ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 Mycobacterium tuberculosis AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.15 additional information biotin-dependent acetyl-CoA carboxylases/transcarboxylases are a class of enzymes that, in addition to fatty-acid biosynthesis, are important for gluconeogenesis as well as propionate catabolism Mycobacterium tuberculosis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.4.15 biotin acetyl-CoA carboxylase ligase
-
 Mycobacterium tuberculosis
6.3.4.15 BirA
-
 Mycobacterium tuberculosis
6.3.4.15 More the enzyme belongs to the biotin-dependent acetyl-CoA carboxylases/transcarboxylases Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.4.15 ATP
-
 Mycobacterium tuberculosis
6.3.4.15 biotin dependent on Mycobacterium tuberculosis