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Literature summary extracted from

  • Tanaka, N.; Aoki, K.; Ishikura, S.; Nagano, M.; Imamura, Y.; Hara, A.; Nakamura, K.T.
    Molecular basis for peroxisomal localization of tetrameric carbonyl reductase (2008), Structure, 16, 388-397.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.184 expression in Escherichia coli Sus scrofa

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.184 C-terminal peroxisomal targeting sequence 1 of each subunit is involved in intersubunit interactions and buried in the interior of the tetrameric enzyme Sus scrofa

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.184 additional information mutation of C-terminal peroxisomal targeting sequence SRL to SKL, SHL, SLL, and SL. Mutants SLL and SL are not catalytically active. Mutant SHL is gradually inactivated during incubation at 0°C. Both mutants SHL and SKL are tetramers and show kinetic parameters similar to wild-type Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.184 peroxisome C-terminal peroxisomal targeting sequence 1 of each subunit is involved in intersubunit interactions and buried in the interior of the tetrameric enzyme Sus scrofa 5777
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.184 27000
-
 4 * 27000, SDS-PAGE and crystallization data Sus scrofa
1.1.1.184 100000
-
 gel filtration, wild-type and mutants SHL, SKL Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.184 Sus scrofa Q8WNV7
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.184 heart
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.184 1-phenylisatin + NADPH
-
 Sus scrofa ? + NADP+
-
 ?
1.1.1.184 4-benzoylpyridine + NADPH + H+
-
 Sus scrofa phenyl-4-pyridylmethanol + NADP+
-
 ?
1.1.1.184 ethyl benzoylformate + NADPH
-
 Sus scrofa ? + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.184 tetramer 4 * 27000, SDS-PAGE and crystallization data Sus scrofa