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Literature summary extracted from

  • McCoy, J.G.; Bailey, L.J.; Ng, Y.H.; Bingman, C.A.; Wrobel, R.; Weber, A.P.; Fox, B.G.; Phillips, G.N.
    Discovery of sarcosine dimethylglycine methyltransferase from Galdieria sulphuraria (2008), Proteins, 74, 368-367.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.157 expression in Escherichia coli Galdieria sulphuraria

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.157 hanging drop vapor-diffusion method, crystal structure of the enzyme, solved to a resolution of 1.95 A Galdieria sulphuraria

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.157 0.144
-
 S-adenosyl-L-methionine cosubstrate: sarcosine Galdieria sulphuraria
2.1.1.157 0.15
-
 S-adenosyl-L-methionine cosubstrate: N,N-dimethylglycine Galdieria sulphuraria
2.1.1.157 2
-
 sarcosine
-
 Galdieria sulphuraria
2.1.1.157 2.8
-
 N,N-dimethylglycine
-
 Galdieria sulphuraria

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.157 34000
-
 2 * 34000, denaturing PAGE Galdieria sulphuraria
2.1.1.157 67700
-
 gel filtration Galdieria sulphuraria

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.157 Galdieria sulphuraria
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.157
-
 Galdieria sulphuraria

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.157 S-adenosyl-L-methionine + N,N-dimethylglycine
-
 Galdieria sulphuraria S-adenosyl-L-homocysteine + betaine
-
 ?
2.1.1.157 S-adenosyl-L-methionine + sarcosine
-
 Galdieria sulphuraria S-adenosyl-L-homocysteine + N,N-dimethylglycine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.157 dimer 2 * 34000, denaturing PAGE Galdieria sulphuraria

Synonyms

EC Number Synonyms Comment Organism
2.1.1.157 dimethylglycine methyltransferase
-
 Galdieria sulphuraria
2.1.1.157 GsSDMT
-
 Galdieria sulphuraria

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.1.157 61
-
 melting temperature Galdieria sulphuraria

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.157 1.07
-
 sarcosine
-
 Galdieria sulphuraria
2.1.1.157 1.4
-
 N,N-dimethylglycine
-
 Galdieria sulphuraria

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.157 7.5
-
 methylation of dimethylglycine Galdieria sulphuraria
2.1.1.157 8
-
 methylation of sarcosine Galdieria sulphuraria