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Literature summary extracted from

  • Martinez-Martinez, I.; Navarro-Fernandez, J.; Garcia-Carmona, F.; Takami, H.; Sanchez-Ferrer, A.
    Characterization and structural modeling of a novel thermostable glycine oxidase from Geobacillus kaustophilus HTA426 (2008), Proteins, 70, 1429-1441.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.19 expressed in Escherichia coli Rosetta (DE3) cells Geobacillus kaustophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.19 0.22
-
 N-ethylglycine in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate, at pH 8.5 and 37°C Geobacillus kaustophilus
1.4.3.19 0.25
-
 glycine in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate, at pH 8.5 and 37°C Geobacillus kaustophilus
1.4.3.19 0.45
-
 sarcosine in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate, at pH 8.5 and 37°C Geobacillus kaustophilus
1.4.3.19 0.52
-
 glycine-ethyl-ester in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate, at pH 8.5 and 37°C Geobacillus kaustophilus
1.4.3.19 9.3
-
 D-proline in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate, at pH 8.5 and 37°C Geobacillus kaustophilus
1.4.3.19 20.6
-
 D-alanine in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate, at pH 8.5 and 37°C Geobacillus kaustophilus
1.4.3.19 221.8
-
 D-pipecolate in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate, at pH 8.5 and 37°C Geobacillus kaustophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.19 40000
-
 4 * 40000, gel filtration Geobacillus kaustophilus
1.4.3.19 43000
-
 4 * 43000, SDS-PAGE Geobacillus kaustophilus
1.4.3.19 160000
-
 gel filtration Geobacillus kaustophilus
1.4.3.19 172000
-
 SDS-PAGE Geobacillus kaustophilus

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.19 Geobacillus kaustophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.19 HiTrap FF chelating affinity column chromatography and phenyl-Sepharose FF hydrophobic column chromatography Geobacillus kaustophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.19 1.75
-
 crude extract, at 37°C and pH 8.5 Geobacillus kaustophilus
1.4.3.19 11.85
-
 after 6.8fold purification, at 37°C and pH 8.5 Geobacillus kaustophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.19 cephalosporin C + H2O + O2
-
 Geobacillus kaustophilus ?
-
 ?
1.4.3.19 D-alanine + H2O + O2
-
 Geobacillus kaustophilus pyruvate + NH3 + H2O2
-
 ?
1.4.3.19 D-arginine + H2O + O2
-
 Geobacillus kaustophilus ?
-
 ?
1.4.3.19 D-aspartate + H2O + O2
-
 Geobacillus kaustophilus ?
-
 ?
1.4.3.19 D-methionine + H2O + O2
-
 Geobacillus kaustophilus 4-(methylsulfanyl)-2-oxobutanoic acid + NH3 + H2O2
-
 ?
1.4.3.19 D-norvaline + H2O + O2
-
 Geobacillus kaustophilus 2-oxopentanoic acid + NH3 + H2O2
-
 ?
1.4.3.19 D-phenylalanine + H2O + O2
-
 Geobacillus kaustophilus phenylpyruvate + NH3 + H2O2
-
 ?
1.4.3.19 D-pipecolate + H2O + O2
-
 Geobacillus kaustophilus ?
-
 ?
1.4.3.19 D-proline + H2O + O2
-
 Geobacillus kaustophilus ?
-
 ?
1.4.3.19 D-valine + H2O + O2
-
 Geobacillus kaustophilus 3-methyl-2-oxobutanoic acid + NH3 + H2O2
-
 ?
1.4.3.19 glycine + H2O + O2
-
 Geobacillus kaustophilus glyoxylate + NH3 + H2O2
-
 ?
1.4.3.19 glycine-ethyl-ester + H2O + O2
-
 Geobacillus kaustophilus ?
-
 ?
1.4.3.19 additional information no activity with butilamine, benzilamine, D-histidine, D-leucine, L-proline, D-tryptophan, and D-tyrosine Geobacillus kaustophilus ?
-
 ?
1.4.3.19 N-ethylglycine + H2O + O2
-
 Geobacillus kaustophilus glyoxylate + ethylamine + H2O2
-
 ?
1.4.3.19 sarcosine + H2O + O2
-
 Geobacillus kaustophilus glyoxylate + methylamine + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.19 homotetramer 4 * 43000, SDS-PAGE Geobacillus kaustophilus
1.4.3.19 homotetramer 4 * 40000, gel filtration Geobacillus kaustophilus

Synonyms

EC Number Synonyms Comment Organism
1.4.3.19 GOXK
-
 Geobacillus kaustophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.3.19 60 70
-
 Geobacillus kaustophilus
1.4.3.19 95
-
 heat treatment of the protein at 95°C for 5 min results in the release of the FAD cofactor Geobacillus kaustophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.19 8.5
-
-
 Geobacillus kaustophilus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.3.19 4 9 a great decrease in stability is only produced at pH values below pH 4.0, shows good stability between pH 6.0 and 9.0 Geobacillus kaustophilus

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.19 FAD flavoenzyme containing noncovalently bound FAD Geobacillus kaustophilus