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Literature summary extracted from

  • Michel, A.A.; Steinhilber, D.; Werz, O.
    Development of a method for expression and purification of the regulatory C2-like domain of human 5-lipoxygenase (2008), Protein Expr. Purif., 59, 110-116.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.11.34 5-LO activating protein i.e. FLAP, Ca2+ induces the translocation of 5-LO from a soluble compartment to nuclear structures, where 5-LO co-localizes with 5-LO activating protein Homo sapiens
1.13.11.34 ATP
-
Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.34 expression of regulatory C2-like domain of the enzyme, residues 1-128, containing a tobacco etch virus protease-cleavage site, in Escherichia coli strain BL21(DE3) as soluble maltose-binding-protein fusion protein Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.34 methanol complete inhibition at 50% v/v Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.13.11.34 cytosol Ca2+ induces the translocation of 5-LO from a soluble compartment to nuclear structures, where 5-LO co-localizes with 5-LO activating protein, FLAP Homo sapiens 5829
-
1.13.11.34 nucleus Ca2+ induces the translocation of 5-LO from a soluble compartment to nuclear structures, where 5-LO co-localizes with 5-LO activating protein, FLAP Homo sapiens 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.34 Ca2+ activates, Ca2+ induces the translocation of 5-LO from a soluble compartment to nuclear structures, where 5-LO co-localizes with 5-LO activating protein, FLAP Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.34 (6E,8Z,11Z,14Z)-(5S)-5-hydroperoxyeicosa-6,8,11,14-tetraenoate Homo sapiens
-
leukotriene A4 + H2O is unstable and metabolized to leukotriene B4 ?
1.13.11.34 arachidonic acid + O2 Homo sapiens
-
(6E,8Z,11Z,14Z)-(5S)-5-hydroperoxyeicosa-6,8,11,14-tetraenoate
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.34 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.13.11.34 phosphoprotein enzyme regulation by phosphorylation Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.34 recombinant soluble MBP-regulatory C2-like domain fusion protein from Escherichia coli strain BL21(DE3) by amylose affinity chromatography, fusion protein cleavage by TEV protease, and further purification of the C2-like domain by hydrophobic interaction chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.34 (6E,8Z,11Z,14Z)-(5S)-5-hydroperoxyeicosa-6,8,11,14-tetraenoate
-
Homo sapiens leukotriene A4 + H2O
-
?
1.13.11.34 (6E,8Z,11Z,14Z)-(5S)-5-hydroperoxyeicosa-6,8,11,14-tetraenoate
-
Homo sapiens leukotriene A4 + H2O is unstable and metabolized to leukotriene B4 ?
1.13.11.34 arachidonic acid + O2
-
Homo sapiens (6E,8Z,11Z,14Z)-(5S)-5-hydroperoxyeicosa-6,8,11,14-tetraenoate
-
?

Subunits

EC Number Subunits Comment Organism
1.13.11.34 More residues 1-128 of the enzyme form the regulatory C2-like domain Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.13.11.34 5-lipoxygenase
-
Homo sapiens
1.13.11.34 5-LO
-
Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.34 37
-
assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.34 7.4
-
assay at Homo sapiens