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Literature summary extracted from

  • Wang, Y.; Ma, X.; Zhao, W.; Jia, X.; Lei, K.; Xu, X.
    Study on the creatinase from Paracoccus sp. strain WB1 (2006), Process Biochem., 41, 2072-2077.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.3.3 phylogenetic tree, overview Paracoccus sp. WB1

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.3.3 Ag+ complete inactivation at 1 mM Paracoccus sp. WB1
3.5.3.3 Co2+ 50% inhibition at 1 mM Paracoccus sp. WB1
3.5.3.3 Cu2+ complete inactivation at 1 mM Paracoccus sp. WB1
3.5.3.3 Hg2+ complete inactivation at 1 mM Paracoccus sp. WB1
3.5.3.3 additional information NaN3, and chelating agents EDTA and 1,10-phenanthroline do not inhibit the activity at the concentration of 20 mM, no inhibition by Tween80 and SDS, and by Ca2+, Pb2+, Ba2+, Fe2+, Fe3+, Mg2+, Cr2+, Mn2+, and Li+ Paracoccus sp. WB1
3.5.3.3 Tween20 strong inhibition at 1 mM Paracoccus sp. WB1
3.5.3.3 Zn2+ 40% inhibition at 1 mM Paracoccus sp. WB1

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.3.3 0.034
-
 Creatine pH 7.5, 37°C Paracoccus sp. WB1

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.3.3 48000
-
 x * 48000, SDS-PAGE Paracoccus sp. WB1

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.3.3 creatine + H2O Paracoccus sp. WB1
-
 sarcosine + urea
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.3 Paracoccus sp. WB1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.3 native enzyme 10.17fold to homogeneity by anion exchange chromatography, hydrophobic interaction chromatography, and gel filtration Paracoccus sp. WB1

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.3.3 additional information
-
 measurement of creatinine in a coupled assay with creatininase and sarcosine oxidase, overview Paracoccus sp. WB1
3.5.3.3 6.1
-
 purified enzyme Paracoccus sp. WB1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.3 creatine + H2O
-
 Paracoccus sp. WB1 sarcosine + urea
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.3.3 ? x * 48000, SDS-PAGE Paracoccus sp. WB1

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.3.3 37
-
-
 Paracoccus sp. WB1

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.3.3 25 50
-
 Paracoccus sp. WB1

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.3.3 25 50 purified enzyme, stable Paracoccus sp. WB1

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.3.3 7.5
-
-
 Paracoccus sp. WB1

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.3.3 5.5 9.5
-
 Paracoccus sp. WB1

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.3.3 5.5 9.5 purified enzyme, stable Paracoccus sp. WB1