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Literature summary extracted from

  • Alfieri, A.; Malito, E.; Orru, R.; Fraaije, M.W.; Mattevi, A.
    Revealing the moonlighting role of NADP in the structure of a flavin-containing monooxygenase (2008), Proc. Natl. Acad. Sci. USA, 105, 6572-6577.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.13.8 mutant E158A/E159A, ligand-free or in complex with NADP+, microbatch technique at 4°C by mixing equal volumes of 8 mg of protein/ml in 25 mM Tris-HCl, pH 8.0, 250 mM NaCl, 1 mM NADP+, and of crystallization solution containing PEG 4000 20% w/v in 0.1 M Na/HEPES, pH 7.5, X-ray diffraction structure determination and analysis at 2.6-2.8 A resolution, molecular replacement Methylophaga aminisulfidivorans

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.8 E158A/E159A the mutant shows similar activity with trimethylamine compared to the wild-type enzyme Methylophaga aminisulfidivorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.8 0.013
-
 NADPH pH 8.5, 25°C Methylophaga aminisulfidivorans
1.14.13.8 0.066
-
 Methimazole pH 8.5, 25°C Methylophaga aminisulfidivorans
1.14.13.8 0.073
-
 trimethylamine pH 8.5, 25°C Methylophaga aminisulfidivorans
1.14.13.8 0.09
-
 indole pH 8.5, 25°C Methylophaga aminisulfidivorans
1.14.13.8 0.13
-
 Nicotine pH 8.5, 25°C Methylophaga aminisulfidivorans
1.14.13.8 0.232
-
 N,N-Dimethylaniline pH 8.5, 25°C Methylophaga aminisulfidivorans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.13.8 soluble
-
 Methylophaga aminisulfidivorans
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.8 53000
-
 2 * 53000, SDS-PAGE Methylophaga aminisulfidivorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.8 N,N-dimethylaniline + NADPH + H+ + O2 Methylophaga aminisulfidivorans
-
 N,N-dimethylaniline N-oxide + NADP+ + H2O
-
 ?
1.14.13.8 N,N-dimethylaniline + NADPH + H+ + O2 Methylophaga aminisulfidivorans SK1
-
 N,N-dimethylaniline N-oxide + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.8 Methylophaga aminisulfidivorans
-
-
-
1.14.13.8 Methylophaga aminisulfidivorans SK1
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.13.8 N,N-dimethylaniline + NADPH + H+ + O2 = N,N-dimethylaniline N-oxide + NADP+ + H2O catalytic cycle, reaction mechanism via C4a-hydroperoxyflavin intermediate and structure-function relationship, overview Methylophaga aminisulfidivorans
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.8 indole + NADPH + H+ + O2
-
 Methylophaga aminisulfidivorans indoxyl + NADP+ + H2O
-
 ?
1.14.13.8 indole + NADPH + H+ + O2
-
 Methylophaga aminisulfidivorans SK1 indoxyl + NADP+ + H2O
-
 ?
1.14.13.8 methimazole + NADPH + H+ + O2
-
 Methylophaga aminisulfidivorans ?
-
 ?
1.14.13.8 methimazole + NADPH + H+ + O2
-
 Methylophaga aminisulfidivorans SK1 ?
-
 ?
1.14.13.8 additional information substrate specificity, the ability to stabilize the hydroperoxyflavin intermediate in substrate oxygenation is crucial involving NADP(H), overview Methylophaga aminisulfidivorans ?
-
 ?
1.14.13.8 additional information substrate specificity, the ability to stabilize the hydroperoxyflavin intermediate in substrate oxygenation is crucial involving NADP(H), overview Methylophaga aminisulfidivorans SK1 ?
-
 ?
1.14.13.8 N,N-dimethylaniline + NADPH + H+ + O2
-
 Methylophaga aminisulfidivorans N,N-dimethylaniline N-oxide + NADP+ + H2O
-
 ?
1.14.13.8 N,N-dimethylaniline + NADPH + H+ + O2
-
 Methylophaga aminisulfidivorans SK1 N,N-dimethylaniline N-oxide + NADP+ + H2O
-
 ?
1.14.13.8 nicotine + NADPH + H+ + O2
-
 Methylophaga aminisulfidivorans nicotine N-oxide + NADP+ + H2O
-
 ?
1.14.13.8 trimethylamine + NADPH + H+ + O2
-
 Methylophaga aminisulfidivorans trimethylamine N-oxide + NADP+ + H2O
-
 ?
1.14.13.8 trimethylamine + NADPH + H+ + O2
-
 Methylophaga aminisulfidivorans SK1 trimethylamine N-oxide + NADP+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.8 dimer 2 * 53000, SDS-PAGE Methylophaga aminisulfidivorans

Synonyms

EC Number Synonyms Comment Organism
1.14.13.8 FMO
-
 Methylophaga aminisulfidivorans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.8 25
-
 assay at Methylophaga aminisulfidivorans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.13.8 0.06
-
 NADPH pH 8.5, 25°C Methylophaga aminisulfidivorans
1.14.13.8 0.7
-
 indole pH 8.5, 25°C Methylophaga aminisulfidivorans
1.14.13.8 1
-
 Methimazole pH 8.5, 25°C Methylophaga aminisulfidivorans
1.14.13.8 1.8
-
 N,N-Dimethylaniline pH 8.5, 25°C Methylophaga aminisulfidivorans
1.14.13.8 3
-
 Nicotine pH 8.5, 25°C Methylophaga aminisulfidivorans
1.14.13.8 6.1
-
 trimethylamine pH 8.5, 25°C Methylophaga aminisulfidivorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.8 8
-
-
 Methylophaga aminisulfidivorans

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.8 FAD binding structure, one molecule of noncovalently tightly bound FAD per enzyme monomer, overview Methylophaga aminisulfidivorans
1.14.13.8 NADPH binding structure, the adjacent ribose of NADP+ is an integral part of the catalytic site being actively engaged in the stabilization of the oxygenating intermediate, overview Methylophaga aminisulfidivorans