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Literature summary extracted from
- Genetics of P450 oxidoreductase: sequence variation in 842 individuals of four ethnicities and activities of 15 missense mutations (2008), Proc. Natl. Acad. Sci. USA, 105, 1733-1738.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.6.2.4 | expression of missense variant proteins lacking 27 N-terminal residues in Escherichia coli | Homo sapiens |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.6.2.4 | A462T | vmax/Km for ferricytochrome c is 85% of the wild-type value. Vmax/Km for NADPH is 69% of the wild-type value | Homo sapiens |
| 1.6.2.4 | A485T | vmax/Km for ferricytochrome c is 36% of the wild-type value. Vmax/Km for NADPH is 51% of the wild-type value | Homo sapiens |
| 1.6.2.4 | A503V | vmax/Km for ferricytochrome c is 67% of the wild-type value. Vmax/Km for NADPH is 56% of the wild-type value | Homo sapiens |
| 1.6.2.4 | D211L | vmax/Km for ferricytochrome c is 27% of the wild-type value. Vmax/Km for NADPH is 59% of the wild-type value | Homo sapiens |
| 1.6.2.4 | delE53 | vmax/Km for ferricytochrome c is 57% of the wild-type value. Vmax/Km for NADPH is 93% of the wild-type value | Homo sapiens |
| 1.6.2.4 | E300K | vmax/Km for ferricytochrome c is 93% of the wild-type value. Vmax/Km for NADPH is 104% of the wild-type value | Homo sapiens |
| 1.6.2.4 | G213E | vmax/Km for ferricytochrome c is 111% of the wild-type value. Vmax/Km for NADPH is 105% of the wild-type value | Homo sapiens |
| 1.6.2.4 | P284L | vmax/Km for ferricytochrome c is 104% of the wild-type value. Vmax/Km for NADPH is 153% of the wild-type value | Homo sapiens |
| 1.6.2.4 | P284T | vmax/Km for ferricytochrome c is 16% of the wild-type value. Vmax/Km for NADPH is 32% of the wild-type value | Homo sapiens |
| 1.6.2.4 | P452L | vmax/Km for ferricytochrome c is 16% of the wild-type value. Vmax/Km for NADPH is 12% of the wild-type value | Homo sapiens |
| 1.6.2.4 | P55L | vmax/Km for ferricytochrome c is 67% of the wild-type value. Vmax/Km for NADPH is 123% of the wild-type value | Homo sapiens |
| 1.6.2.4 | R406H | vmax/Km for ferricytochrome c is 62% of the wild-type value. Vmax/Km for NADPH is 78% of the wild-type value | Homo sapiens |
| 1.6.2.4 | R600W | vmax/Km for ferricytochrome c is 18% of the wild-type value. Vmax/Km for NADPH is 7% of the wild-type value | Homo sapiens |
| 1.6.2.4 | V472M | vmax/Km for ferricytochrome c is 23% of the wild-type value. Vmax/Km for NADPH is 24% of the wild-type value | Homo sapiens |
| 1.6.2.4 | Y607C | Vmax/Km for NADPH is 20% of the wild-type value | Homo sapiens |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.6.2.4 | 0.025 | - |
NADPH | mutant enzyme Y607C | Homo sapiens |  |
| 1.6.2.4 | 0.066 | - |
NADPH | mutant enzyme P284T | Homo sapiens | |
| 1.6.2.4 | 0.084 | - |
NADPH | mutant enzyme R600W | Homo sapiens | |
| 1.6.2.4 | 0.125 | - |
NADPH | mutant enzyme P452L | Homo sapiens | |
| 1.6.2.4 | 0.144 | - |
NADPH | wild-type enzyme | Homo sapiens | |
| 1.6.2.4 | 0.144 | - |
NADPH | mutant enzyme A485T | Homo sapiens | |
| 1.6.2.4 | 0.146 | - |
NADPH | mutant enzyme P55L | Homo sapiens | |
| 1.6.2.4 | 0.151 | - |
NADPH | mutant enzyme delE53 | Homo sapiens | |
| 1.6.2.4 | 0.153 | - |
NADPH | mutant enzyme D211L | Homo sapiens | |
| 1.6.2.4 | 0.155 | - |
NADPH | mutant enzyme A503V | Homo sapiens | |
| 1.6.2.4 | 0.157 | - |
NADPH | mutant enzyme P284L | Homo sapiens | |
| 1.6.2.4 | 0.169 | - |
NADPH | mutant enzyme V472M | Homo sapiens | |
| 1.6.2.4 | 0.177 | - |
NADPH | mutant enzyme R406H | Homo sapiens | |
| 1.6.2.4 | 0.196 | - |
NADPH | mutant enzyme G213E | Homo sapiens | |
| 1.6.2.4 | 0.222 | - |
NADPH | mutant enzyme E300K | Homo sapiens | |
| 1.6.2.4 | 0.276 | - |
NADPH | mutant enzyme A462T | Homo sapiens | |
| 1.6.2.4 | 0.39 | - |
ferricytochrome c | mutant enzyme R600W | Homo sapiens | |
| 1.6.2.4 | 0.92 | - |
ferricytochrome c | mutant enzyme P452L | Homo sapiens | |
| 1.6.2.4 | 0.99 | - |
ferricytochrome c | mutant enzyme A503V | Homo sapiens | |
| 1.6.2.4 | 1.15 | - |
ferricytochrome c | mutant enzyme P284T | Homo sapiens | |
| 1.6.2.4 | 1.19 | - |
ferricytochrome c | wild-type enzyme | Homo sapiens | |
| 1.6.2.4 | 1.37 | - |
ferricytochrome c | mutant enzyme V472M | Homo sapiens | |
| 1.6.2.4 | 1.61 | - |
ferricytochrome c | mutant enzyme G213E | Homo sapiens | |
| 1.6.2.4 | 1.84 | - |
ferricytochrome c | mutant enzyme A485T | Homo sapiens | |
| 1.6.2.4 | 1.86 | - |
ferricytochrome c | mutant enzyme E300K | Homo sapiens | |
| 1.6.2.4 | 2.07 | - |
ferricytochrome c | mutant enzyme R406H | Homo sapiens | |
| 1.6.2.4 | 2.09 | - |
ferricytochrome c | mutant enzyme A462T | Homo sapiens | |
| 1.6.2.4 | 2.16 | - |
ferricytochrome c | mutant enzyme delE53 | Homo sapiens | |
| 1.6.2.4 | 2.25 | - |
ferricytochrome c | mutant enzyme P284L | Homo sapiens | |
| 1.6.2.4 | 2.31 | - |
ferricytochrome c | mutant enzyme P55L | Homo sapiens | |
| 1.6.2.4 | 2.5 | 5 | ferricytochrome c | mutant enzyme D211L | Homo sapiens | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.6.2.4 | Homo sapiens | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.6.2.4 | 2 ferricytochrome c + NADPH | - |
Homo sapiens | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.6.2.4 | P450 oxidoreductase | - |
Homo sapiens |
| 1.6.2.4 | POR | - |
Homo sapiens |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.6.2.4 | NADPH | - |
Homo sapiens | |
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