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Literature summary extracted from

  • Lin, Y.F.; Walmsley, A.R.; Rosen, B.P.
    An arsenic metallochaperone for an arsenic detoxification pump (2006), Proc. Natl. Acad. Sci. USA, 103, 15617-15622.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
7.3.2.7 ArsD encoded in the arsRDABC operon, the dimeric arsenic chaperone ArsD transfers trivalent metalloids to ArsA, the catalytic subunit of an As(III)-Sb(III) efflux pump. Interaction with ArsD increases the affinity of ArsA for arsenite and antimonite , thus increasing its ATPase activity at lower concentrations of arsenite 60fold, as well as for antimonite, and enhancing the rate of arsenite extrusion rendering cells resistant to environmental concentrations of arsenic, overview, cannot be replaced by DTT Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.3.2.7 expression of ArsA and ArsD wild-type and mutants in a Saccharomyces cerevisiae two-hybrid system for interaction analysis, overview Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.3.2.7 As3+ stimulates Escherichia coli
7.3.2.7 Mg2+ as MgATP2- Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.3.2.7 ATP + H2O + antimonite/in Escherichia coli the arsenic chaperone ArsD transfers trivalent metalloids to ArsA, the catalytic subunit of an As(III)-Sb(III) efflux pump. Interaction with ArsD increases the affinity of ArsA for antimonite, thus increasing its ATPase activity at lower concentrations of antimonite, regulatory mechanism, overview ADP + phosphate + antimonite/out
-
 ?
7.3.2.7 ATP + H2O + arsenite/in Escherichia coli the arsenic chaperone ArsD transfers trivalent metalloids to ArsA, the catalytic subunit of an As(III)-Sb(III) efflux pump. Interaction with ArsD increases the affinity of ArsA for arsenite, thus increasing its ATPase activity at lower concentrations of arsenite and enhancing the rate of arsenite extrusion rendering cells resistant to environmental concentrations of arsenic, regulatory mechanism, overview ADP + phosphate + arsenite/out
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.3.2.7 Escherichia coli
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 gene arsA, encoded in the arsRDABC operon
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.3.2.7 ATP + H2O + antimonite/in the arsenic chaperone ArsD transfers trivalent metalloids to ArsA, the catalytic subunit of an As(III)-Sb(III) efflux pump. Interaction with ArsD increases the affinity of ArsA for antimonite, thus increasing its ATPase activity at lower concentrations of antimonite, regulatory mechanism, overview Escherichia coli ADP + phosphate + antimonite/out
-
 ?
7.3.2.7 ATP + H2O + arsenite/in
-
 Escherichia coli ADP + phosphate + arsenite/out
-
 ?
7.3.2.7 ATP + H2O + arsenite/in the arsenic chaperone ArsD transfers trivalent metalloids to ArsA, the catalytic subunit of an As(III)-Sb(III) efflux pump. Interaction with ArsD increases the affinity of ArsA for arsenite, thus increasing its ATPase activity at lower concentrations of arsenite and enhancing the rate of arsenite extrusion rendering cells resistant to environmental concentrations of arsenic, regulatory mechanism, overview Escherichia coli ADP + phosphate + arsenite/out
-
 ?
7.3.2.7 additional information ArsA specifically interacts with ArsD but not with ArsR or ArsC Escherichia coli ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.3.2.7 arsenic detoxification pump
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 Escherichia coli