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Literature summary extracted from

  • Kuntz Angela, N.; Davioud-Charvet, E.; Sayed Ahmed, A.; Califf Lindsay, L.; Dessolin, J.; Arner Elias, S.J.; Williams David, L.
    Thioredoxin glutathione reductase from Schistosoma mansoni: an essential parasite enzyme and a key drug target (2007), PLoS Med., 4, e206.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.8.1.B1 medicine TGR is a key target for antischistosomal drug development Schistosoma mansoni

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.B1 3-DAP 0.05 mM, complete inhibition Schistosoma mansoni
1.8.1.B1 auranofin 0.05 mM, complete inhibition Schistosoma mansoni
1.8.1.B1 aurothioglucose 0.05 mM, complete inhibition Schistosoma mansoni
1.8.1.B1 aurothiomalate 0.05 mM, complete inhibition Schistosoma mansoni
1.8.1.B1 CDE16-2 0.05 mM, 98% inhibition Schistosoma mansoni
1.8.1.B1 CDE4 0.05 mM, 99% inhibition Schistosoma mansoni
1.8.1.B1 JD159 0.05 mM, complete inhibition Schistosoma mansoni
1.8.1.B1 LS826 0.05 mM, 95% inhibition Schistosoma mansoni
1.8.1.B1 naphthazarin 0.05 mM, complete inhibition Schistosoma mansoni
1.8.1.B1 OPZ 0.05 mM, 87% inhibition Schistosoma mansoni
1.8.1.B1 PAT 0.05 mM, complete inhibition Schistosoma mansoni
1.8.1.B1 RMA35 0.05 mM, 98% inhibition Schistosoma mansoni

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.B1 0.0137
-
 NADPH pH 7.4, 25°C Schistosoma mansoni
1.8.1.B1 0.0715
-
 GSSG pH 7.4, 25°C Schistosoma mansoni
1.8.1.B1 0.114
-
 5,5'-dithiobis(2-nitrobenzoic acid) pH 7.4, 25°C Schistosoma mansoni

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.B1 Schistosoma mansoni
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.B1
-
 Schistosoma mansoni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.B1 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+
-
 Schistosoma mansoni 2-nitro-5-thiobenzoate + NADP+
-
 ?
1.8.1.B1 GSSG + NADPH + H+
-
 Schistosoma mansoni GSH + NADP+
-
 ?
1.8.1.B1 thioredoxin + NADP+
-
 Schistosoma mansoni thioredoxin disulfide + NADPH
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.1.B1 TGR
-
 Schistosoma mansoni

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.1.B1 16
-
 5,5'-dithiobis(2-nitrobenzoic acid) pH 7.4, 25°C Schistosoma mansoni
1.8.1.B1 20.1
-
 NADPH pH 7.4, 25°C Schistosoma mansoni
1.8.1.B1 21.7
-
 GSSG pH 7.4, 25°C Schistosoma mansoni

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.8.1.B1 0.00001
-
 auranofin
-
 Schistosoma mansoni

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.8.1.B1 0.000007
-
 pH 7.4, 25°C, substrates: 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH Schistosoma mansoni auranofin
1.8.1.B1 0.000009
-
 pH 7.4, 25°C, substrates: GSSG + NADPH Schistosoma mansoni auranofin
1.8.1.B1 0.000037
-
 pH 7.4, 25°C, substrates: GSSG + NADPH Schistosoma mansoni PAT
1.8.1.B1 0.00005
-
 pH 7.4, 25°C, substrates: GSSG + NADPH Schistosoma mansoni aurothiomalate
1.8.1.B1 0.000051
-
 pH 7.4, 25°C, substrates: 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH Schistosoma mansoni PAT
1.8.1.B1 0.00007
-
 pH 7.4, 25°C, substrates: 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH Schistosoma mansoni aurothioglucose
1.8.1.B1 0.00009
-
 pH 7.4, 25°C, substrates: 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH Schistosoma mansoni aurothiomalate
1.8.1.B1 0.0005
-
 pH 7.4, 25°C, substrates: 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH Schistosoma mansoni 3-DAP
1.8.1.B1 0.0005
-
 pH 7.4, 25°C, substrates: 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH Schistosoma mansoni JD159
1.8.1.B1 0.0005
-
 pH 7.4, 25°C, substrates: GSSG + NADPH Schistosoma mansoni CDE4
1.8.1.B1 0.001
-
 pH 7.4, 25°C, substrates: GSSG + NADPH Schistosoma mansoni CDE16-2
1.8.1.B1 0.001
-
 pH 7.4, 25°C, substrates: GSSG + NADPH Schistosoma mansoni JD159
1.8.1.B1 0.0025
-
 pH 7.4, 25°C, substrates: 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH Schistosoma mansoni naphthazarin
1.8.1.B1 0.003
-
 pH 7.4, 25°C, substrates: GSSG + NADPH Schistosoma mansoni aurothioglucose
1.8.1.B1 0.004
-
 pH 7.4, 25°C, substrates: 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH Schistosoma mansoni RMA35
1.8.1.B1 0.006
-
 pH 7.4, 25°C, substrates: GSSG + NADPH Schistosoma mansoni RMA35
1.8.1.B1 0.007
-
 pH 7.4, 25°C, substrates: 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH Schistosoma mansoni CDE4
1.8.1.B1 0.007
-
 pH 7.4, 25°C, substrates: GSSG + NADPH Schistosoma mansoni 3-DAP
1.8.1.B1 0.008
-
 pH 7.4, 25°C, substrates: 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH Schistosoma mansoni LS826
1.8.1.B1 0.008
-
 pH 7.4, 25°C, substrates: GSSG + NADPH Schistosoma mansoni LS826
1.8.1.B1 0.01
-
 pH 7.4, 25°C, substrates: GSSG + NADPH Schistosoma mansoni naphthazarin
1.8.1.B1 0.025
-
 pH 7.4, 25°C, substrates: 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH Schistosoma mansoni CDE16-2
1.8.1.B1 0.05
-
 pH 7.4, 25°C, substrates: GSSG + NADPH Schistosoma mansoni OPZ
1.8.1.B1 0.08
-
 pH 7.4, 25°C, substrates: 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH Schistosoma mansoni OPZ