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Literature summary extracted from

  • Bruneau, L.; Chapman, R.; Marsolais, F.
    Co-occurrence of both L-asparaginase subtypes in Arabidopsis: At3g16150 encodes a K+-dependent L-asparaginase (2006), Planta, 224, 668-679.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.1 expression in Escherichia coli as N-terminal His-tagged protein Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.1 K+ catalytic activity of At3g16150 is enhanced approximately tenfold in the presence of K+ Arabidopsis thaliana
3.5.1.1 Na+ monovalent cation preference in order of decreasing efficiency is: K+, Na+, Rb+ Arabidopsis thaliana
3.5.1.1 Rb+ monovalent cation preference in order of decreasing efficiency is: K+, Na+, Rb+ Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.1 14200
-
 x * 27000 + x * 14200, SDS-PAGE Arabidopsis thaliana
3.5.1.1 27000
-
 x * 27000 + x * 14200, SDS-PAGE Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.1 Arabidopsis thaliana Q8GXG1 ecotype Columbia
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.1 flower activity is highest in sink tissues, especially in flowers and siliques Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.1 L-asparagine + H2O strictly specific for L-Asn, has no activity towards beta-aspartyl dipeptides Arabidopsis thaliana L-aspartate + NH3
-
 ?
3.5.1.1 L-aspartyl hydroxamate + H2O
-
 Arabidopsis thaliana L-aspartate + hydroxylamine
-
 ?
3.5.1.1 additional information not active with N-acetylglucosaminyl-L-Asn Arabidopsis thaliana ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.1 ? x * 27000 + x * 14200, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
3.5.1.1 At3g16150
-
 Arabidopsis thaliana