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Literature summary extracted from

  • Barranco-Medina, S.; Krell, T.; Finkemeier, I.; Sevilla, F.; Lazaro, J.J.; Dietz, K.J.
    Biochemical and molecular characterization of the mitochondrial peroxiredoxin PsPrxII F from Pisum sativum (2007), Plant Physiol. Biochem., 45, 729-739.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.11.1.24 Cd2+ stimulates enzyme expression Pisum sativum
1.11.1.24 Na+ Na+ at 25 mM stimulates expression of PrxII F Pisum sativum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.24 expressed in Escherichia coli BL21 (DE3) cells Pisum sativum

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.24 C48S 12% activity compared to the wild type enzyme Pisum sativum
1.11.1.24 C59S inactive Pisum sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.24 0.0186
-
 H2O2 wild type enzyme, in 25 mM Tris-HCl (pH 8) Pisum sativum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.11.1.24 mitochondrion
-
 Pisum sativum 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.24 18570
-
 estimated from amino acid sequence Pisum sativum
1.11.1.24 18750
-
 mature enzyme, gel filtration Pisum sativum
1.11.1.24 20000
-
 2 * 20000, SDS-PAGE Pisum sativum
1.11.1.24 40000
-
 SDS-PAGE Pisum sativum

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.24 Pisum sativum A0ZXY5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.24
-
 Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.1.24 leaf
-
 Pisum sativum
-
1.11.1.24 root
-
 Pisum sativum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.24 H2O2 + reduced thioredoxin
-
 Pisum sativum H2O + oxidized thioredoxin
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.24 dimer 2 * 20000, SDS-PAGE Pisum sativum

Synonyms

EC Number Synonyms Comment Organism
1.11.1.24 PrxII F
-
 Pisum sativum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.24 10.6
-
 H2O2 wild type enzyme, in 25 mM Tris-HCl (pH 8) Pisum sativum

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.24 thioredoxin
-
 Pisum sativum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.11.1.24 Pisum sativum estimated from amino acid sequence
-
 5.92