EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.19.4 | expression in Saccharomyces cerevisiae confers tolerance to aluminium to the cells | Arabidopsis thaliana |
1.14.19.4 | gene S581, DNA and amino acid sequence determination and analysis, expression in Saccharomyces cerevisiae and constitutively in Arabidopsis thaliana, under control of the CaMV35S promoter, confers tolerance to aluminium and gadolinium, Gd3+, but not to lanthanum, La3+, or manganese, Mn2+, to cells and seedlings, respectively, the transgenic expression leads to the accumulation of 8(Z/E)-C18-phytosphingenine and 8(Z/E)-C20-phytopshingenine in yeast and to the accumulation of 8(Z/E)-C18-phytosphingenine in the leaves and roots of Arabidopsis plants, overview | Stylosanthes hamata |
1.14.19.29 | expressed in Saccharomyces cerevisiae and Arabidopsis thaliana | Stylosanthes hamata |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.19.4 | DELTA4-sphingenine + reduced acceptor + O2 | Stylosanthes hamata | - |
DELTA4,8-sphingadienine + acceptor + 2 H2O | production of two water molecules in the reaction | ? | |
1.14.19.4 | phytosphinganine + reduced acceptor + O2 | Stylosanthes hamata | - |
DELTA8-phytosphingenine + acceptor + H2O | production of two water molecules in the reaction | ? | |
1.14.19.4 | sphinganine + reduced acceptor + O2 | Stylosanthes hamata | - |
DELTA8-sphingenine + acceptor + 2 H2O | production of two water molecules in the reaction | ? | |
1.14.19.29 | (4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+ | Stylosanthes hamata | - |
(4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O | - |
? | |
1.14.19.29 | (4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+ | Stylosanthes hamata | - |
(4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O | the enzyme exhibits preference for (Z)-isomer formation | ? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.19.4 | Arabidopsis thaliana | AF001394 | - |
- |
1.14.19.4 | Stylosanthes hamata | A7Y7E1 | gene S581 | - |
1.14.19.29 | Stylosanthes hamata | A7Y7E1 | - |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.19.4 | root | - |
Stylosanthes hamata | - |
1.14.19.29 | root | - |
Stylosanthes hamata | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.19.4 | DELTA4-sphingenine + reduced acceptor + O2 | - |
Stylosanthes hamata | DELTA4,8-sphingadienine + acceptor + 2 H2O | production of two water molecules in the reaction | ? | |
1.14.19.4 | additional information | DELTA8 sphingolipid desaturase creates double bonds in long-chain bases, i.e. sphingobases, such as sphinganine, phytosphinganine, or 4-sphingenine to produce E- and Z-isomers of 8-sphingenine, 8-phytosphingenine, or 4,8-sphingadienine, respectively | Stylosanthes hamata | ? | - |
? | |
1.14.19.4 | phytosphinganine + reduced acceptor + O2 | - |
Stylosanthes hamata | DELTA8-phytosphingenine + acceptor + H2O | production of two water molecules in the reaction | ? | |
1.14.19.4 | sphinganine + reduced acceptor + O2 | - |
Stylosanthes hamata | DELTA8-sphingenine + acceptor + 2 H2O | production of two water molecules in the reaction | ? | |
1.14.19.29 | (4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+ | - |
Stylosanthes hamata | (4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O | - |
? | |
1.14.19.29 | (4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+ | - |
Stylosanthes hamata | (4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O | the enzyme exhibits preference for (Z)-isomer formation | ? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.19.4 | DELTA8 sphingobase desaturase | - |
Stylosanthes hamata |
1.14.19.4 | DELTA8 sphingobase desaturase | - |
Arabidopsis thaliana |
1.14.19.4 | Delta8 sphingolipid desaturase | - |
Stylosanthes hamata |
1.14.19.4 | Delta8 sphingolipid desaturase | - |
Arabidopsis thaliana |
1.14.19.29 | Delta8 sphingolipid desaturase | - |
Stylosanthes hamata |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.19.29 | cytochrome b5 | - |
Stylosanthes hamata |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.19.29 | physiological function | DELTA8 sphingobase desaturase confer aluminum tolerance in seedlings | Stylosanthes hamata |