EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.1.111 | expressed in Escherichia coli, BL21 cells for induction of recombinant protein expression | Ruta graveolens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.1.1.111 | N298E | generated by site-directed mutagenesis | Ruta graveolens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.111 | 0.003 | - |
S-adenosyl-L-methionine | wild-type | Ruta graveolens | |
2.1.1.111 | 0.003 | - |
S-adenosyl-L-methionine | N298E mutant | Ruta graveolens | |
2.1.1.111 | 0.007 | - |
anthranilate | wild-type | Ruta graveolens | |
2.1.1.111 | 0.024 | - |
anthranilate | N298E mutant | Ruta graveolens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.111 | Ca2+ | 73% relative enzyme activity in presence of 1.5 mM, significantly reduced | Ruta graveolens | |
2.1.1.111 | Co2+ | activity reduced in presence of 1.5 mM, 25% relative activity retained | Ruta graveolens | |
2.1.1.111 | Cu2+ | activity abolished in presence of 1.5 mM of | Ruta graveolens | |
2.1.1.111 | Fe2+ | activity nearly abolished in presence of 1.5 mM of | Ruta graveolens | |
2.1.1.111 | Fe3+ | activity abolished in presence of 1.5 mM of | Ruta graveolens | |
2.1.1.111 | Mg2+ | activity independent in presence of 1.5 mM of, 90% relative activity retained | Ruta graveolens | |
2.1.1.111 | Zn2+ | activity abolished in presence of 1.5 mM of | Ruta graveolens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.1.111 | 70000 | - |
gel filtration, SDS-PAGE | Ruta graveolens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.111 | S-adenosyl-L-methionine + anthranilate | Ruta graveolens | first specific step in acridone alkaloid biosynthesis | S-adenosyl-L-homocysteine + N-methylanthranilate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.111 | Ruta graveolens | A9X7L0 | line R-20 | - |
2.3.1.159 | Ruta graveolens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.1.111 | gel filtration, SDS-PAGE, wild-type and mutant protein | Ruta graveolens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.1.1.111 | carpel | lining the inside of | Ruta graveolens | - |
2.1.1.111 | cell suspension culture | crude extracts of total plants, elicited, 2 years old | Ruta graveolens | - |
2.1.1.111 | flower | high mRNA expression and enzyme activity | Ruta graveolens | - |
2.1.1.111 | leaf | moderate mRNA expression | Ruta graveolens | - |
2.1.1.111 | ovary | central vascular bundles of, around cup-like structures in the outer ovary wall representing secretory oil glands | Ruta graveolens | - |
2.1.1.111 | root | rhizodermis of older root parts, endodermis of younger root parts, high mRNA expression and enzyme activity | Ruta graveolens | - |
2.1.1.111 | stem | vascular region of secondary and primary shoots, moderate mRNA expression, high enzyme activity | Ruta graveolens | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.1.1.111 | additional information | - |
in-gel digestion and peptide sequencing by mass spectrometry, Western Blot and phylogenetic analysis, assays conducted at 32°C in 73.5 mM glycine-NaOH buffer at pH 8.9, HPLC separation of assay extracts, list of compounds that are not accepted as substrate in assay | Ruta graveolens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.111 | S-adenosyl-L-methionine + anthranilate | first specific step in acridone alkaloid biosynthesis | Ruta graveolens | S-adenosyl-L-homocysteine + N-methylanthranilate | - |
? | |
2.1.1.111 | S-adenosyl-L-methionine + anthranilate | first specific step in alkaloid biosynthesis, substrate specificity determines the course of acridone biosynthesis | Ruta graveolens | S-adenosyl-L-homocysteine + N-methylanthranilate | - |
? | |
2.3.1.159 | N-methylanthraniloyl-CoA + 3 malonyl-CoA | anthraniloyl CoA is not accepted as starter substrate, the anthranilate N-methylation prior to CoA activation is a key step in acridone alkaloid formation, anthranilate N-methyltransferase is a branch-point enzyme of acridone biosynthesis | Ruta graveolens | 1,3-dihydroxy-N-methylacridone + CoA + CO2 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.1.111 | ANMT | - |
Ruta graveolens |
2.3.1.159 | acridone synthase | - |
Ruta graveolens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.1.111 | 32 | - |
assay at | Ruta graveolens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.1.1.111 | 8 | - |
activity optimum at | Ruta graveolens |