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Literature summary extracted from

  • Heim, W.G.; Sykes, K.A.; Hildreth, S.B.; Sun, J.; Lu, R.H.; Jelesko, J.G.
    Cloning and characterization of a Nicotiana tabacum methylputrescine oxidase transcript (2006), Phytochemistry, 68, 454-463.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.22 expressed in Escherichia coli Rosetta cells as a recombinant thioredoxin-His6-MPO1 fusion protein, using the plasmid pJGJ389. The recombinant MPO1 protein utilizes N-methylputrescine more efficiently than other diamines, a homology-based cloning strategy Nicotiana tabacum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.22 additional information
-
 additional information the kinetic properties of the MPO1 enzyme may play an important role in determining the pyridine alkaloid profiles observed in tobacco roots Nicotiana tabacum
1.4.3.22 0.19
-
 N-methylputrescine Vmax: 28.8 nkat, pH and temperature not specified in the publication Nicotiana tabacum
1.4.3.22 0.35
-
 1,3-diaminopropane Vmax: 11.3 nkat, pH and temperature not specified in the publication Nicotiana tabacum
1.4.3.22 0.76
-
 1,4-diaminobutane Vmax: 11.1 nkat, pH and temperature not specified in the publication Nicotiana tabacum
1.4.3.22 1.79
-
 cadaverine Vmax: 5.2 nkat, pH and temperature not specified in the publication Nicotiana tabacum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.22 106000
-
 thioredoxin-His6-MPO1 fusion protein Nicotiana tabacum

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.22 Nicotiana tabacum
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.22 hairy root culture
-
 Nicotiana tabacum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.22 1,3-diaminopropane + H2O + O2
-
 Nicotiana tabacum 3-aminopropanal + NH3 + H2O2
-
 ?
1.4.3.22 1,4-diaminobutane + H2O + O2
-
 Nicotiana tabacum 4-aminobutanal + NH3 + H2O2
-
 ?
1.4.3.22 cadaverine + H2O + O2
-
 Nicotiana tabacum 5-aminopentanal + NH3 + H2O2
-
 ?
1.4.3.22 N-methylputrescine + H2O + O2
-
 Nicotiana tabacum 4-(methylamino)butanal + NH3 + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.4.3.22 methylputrescine oxidase
-
 Nicotiana tabacum
1.4.3.22 MPO
-
 Nicotiana tabacum
1.4.3.22 pWGH15
-
 Nicotiana tabacum

General Information

EC Number General Information Comment Organism
1.4.3.22 physiological function the oxidative deamination is an essential step in both pyridine and tropane alkaloid biosynthesis. MPO is part of a multigene family Nicotiana tabacum