EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.73 | expressed in Escherichia coli BL21(DE3)-RIL cells | Methanocaldococcus jannaschii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.1.1.27 | additional information | - |
additional information | steady-state and single-turnover kinetics, kinetic analysis, overview | Methanocaldococcus jannaschii | |
6.1.1.27 | 0.00097 | - |
m1G37-tRNACys | 60°C, pH 6.0, steady-state kinetics | Methanocaldococcus jannaschii | |
6.1.1.27 | 0.0011 | - |
tRNACys | 60°C, pH 6.0, steady-state kinetics | Methanocaldococcus jannaschii | |
6.1.1.27 | 0.007 | - |
tRNACys | pH 7.5, 37°C, recombinant enzyme, native tRNACys | Methanocaldococcus jannaschii | |
6.1.1.27 | 0.0097 | - |
tRNACys | pH 7.5, 37°C, recombinant enzyme, m1G37 tRNACys | Methanocaldococcus jannaschii |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
6.1.1.27 | Mg2+ | - |
Methanocaldococcus jannaschii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.73 | O-phospho-L-seryl-tRNACys + sulfide | Methanocaldococcus jannaschii | Methanocaldococcus jannaschii synthesizes Cys-tRNACys by an indirect pathway, whereby O-phosphoseryltRNA synthetase (SepRS) acylates tRNACys with phosphoserine (Sep), and Sep-tRNACys-tRNA synthase (SepCysS) converts the tRNA-bound phosphoserine to cysteine | L-cysteinyl-tRNACys + phosphate | - |
? | |
6.1.1.27 | ATP + O-phospho-L-serine + tRNACys | Methanocaldococcus jannaschii | - |
AMP + diphosphate + O-phospho-L-seryl-tRNACys | - |
? | |
6.1.1.27 | ATP + O-phospho-L-serine + tRNACys | Methanocaldococcus jannaschii | Methanocaldococcus jannaschii synthesizes Cys-tRNACys by an indirect pathway, whereby O-phosphoseryltRNA synthetase (SepRS) acylates tRNACys with phosphoserine (Sep), and Sep-tRNACys-tRNA synthase (SepCysS) converts the tRNA-bound phosphoserine to cysteine | AMP + diphosphate + O-phospho-L-seryl-tRNACys | - |
? | |
6.1.1.27 | additional information | Methanocaldococcus jannaschii | Methanocaldococcus jannaschii synthesizes Cys-tRNACys by an indirect pathway, whereby O-phosphoseryl-tRNA synthetase acylates tRNACys with phosphoserine, and Sep-tRNA-Cys-tRNA synthase converts the tRNA-bound phosphoserine to cysteine. Methanocaldococcus jannaschii SepRS differs from CysRS by recruiting the m1G37 modification as a determinant for aminoacylation | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.73 | Methanocaldococcus jannaschii | - |
- |
- |
2.5.1.73 | Methanocaldococcus jannaschii | Q59072 | - |
- |
6.1.1.16 | Escherichia coli | - |
- |
- |
6.1.1.27 | Methanocaldococcus jannaschii | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.73 | Q Sepharose FF column chromatography and FPLC Mono Q column chromatography | Methanocaldococcus jannaschii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.73 | O-phospho-L-seryl-tRNACys + sulfide | - |
Methanocaldococcus jannaschii | L-cysteinyl-tRNACys + phosphate | - |
? | |
2.5.1.73 | O-phospho-L-seryl-tRNACys + sulfide | Methanocaldococcus jannaschii synthesizes Cys-tRNACys by an indirect pathway, whereby O-phosphoseryltRNA synthetase (SepRS) acylates tRNACys with phosphoserine (Sep), and Sep-tRNACys-tRNA synthase (SepCysS) converts the tRNA-bound phosphoserine to cysteine | Methanocaldococcus jannaschii | L-cysteinyl-tRNACys + phosphate | - |
? | |
2.5.1.73 | O-phospho-L-seryl-tRNACys + sulfide | Methanocaldococcus jannaschii synthesizes Cys-tRNACys by an indirect pathway, whereby O-phosphoseryltRNA synthetase (SepRS) acylates tRNACys with phosphoserine (Sep), and Sep-tRNACys-tRNA synthase (SepCysS) converts the tRNA-bound phosphoserine to cysteine. O-PhosphoseryltRNA synthetase and Sep-tRNACys-tRNA synthase bind the reaction intermediate O-phospho-L-serine-tRNACys tightly, and these two enzymes form a stable binary complex that promotes conversion of the intermediate to the product and sequesters the intermediate from binding to elongation factor EF-1a or infiltrating into the ribosome | Methanocaldococcus jannaschii | L-cysteinyl-tRNACys + phosphate | - |
? | |
6.1.1.16 | ATP + L-cysteine + tRNACys | - |
Escherichia coli | AMP + diphosphate + L-cysteinyl-tRNACys | - |
? | |
6.1.1.16 | ATP + L-cysteine + tRNACysA36G | relative activity compared to wild-type tRNACys as a substrate: 0.01 | Escherichia coli | AMP + diphosphate + L-cysteinyl-tRNACysA36G | - |
? | |
6.1.1.16 | ATP + L-cysteine + tRNACysC35U | relative activity compared to wild-type tRNACys as a substrate: 0.005 | Escherichia coli | AMP + diphosphate + L-cysteinyl-tRNACysC35U | - |
? | |
6.1.1.16 | ATP + L-cysteine + tRNACysG15C/C48G | relative activity compared to wild-type tRNACys as a substrate: 0.03 | Escherichia coli | AMP + diphosphate + L-cysteinyl-tRNACysG15C/C48G | - |
? | |
6.1.1.16 | ATP + L-cysteine + tRNACysG34C | relative activity compared to wild-type tRNACys as a substrate: 0.001 | Escherichia coli | AMP + diphosphate + L-cysteinyl-tRNACysG34C | - |
? | |
6.1.1.16 | ATP + L-cysteine + tRNACysU73G | relative activity compared to wild-type tRNACys as a substrate: 0.00002 | Escherichia coli | AMP + diphosphate + L-cysteinyl-tRNACysU73G | - |
? | |
6.1.1.27 | ATP + O-phospho-L-serine + m1G37-tRNACys | - |
Methanocaldococcus jannaschii | AMP + diphosphate + O-phospho-L-seryl-m1G37-tRNACys | - |
? | |
6.1.1.27 | ATP + O-phospho-L-serine + tRNACys | - |
Methanocaldococcus jannaschii | AMP + diphosphate + O-phospho-L-seryl-tRNACys | - |
? | |
6.1.1.27 | ATP + O-phospho-L-serine + tRNACys | Methanocaldococcus jannaschii synthesizes Cys-tRNACys by an indirect pathway, whereby O-phosphoseryltRNA synthetase (SepRS) acylates tRNACys with phosphoserine (Sep), and Sep-tRNACys-tRNA synthase (SepCysS) converts the tRNA-bound phosphoserine to cysteine | Methanocaldococcus jannaschii | AMP + diphosphate + O-phospho-L-seryl-tRNACys | - |
? | |
6.1.1.27 | ATP + O-phospho-L-serine + tRNACys | SepRS differs from CysRS (EC 6.1.1.16) by recruiting the m1G37 modification as a determinant for aminoacylation, and in showing limited discrimination against mutations of conserved nucleotides. O-PhosphoseryltRNA synthetase and Sep-tRNACys-tRNA synthase bind the reaction intermediate O-phospho-L-serine-tRNACys tightly, and these two enzymes form a stable binary complex that promotes conversion of the intermediate to the product and sequesters the intermediate from binding to elongation factor EF-1a or infiltrating into the ribosome | Methanocaldococcus jannaschii | AMP + diphosphate + O-phospho-L-seryl-tRNACys | - |
? | |
6.1.1.27 | ATP + O-phospho-L-serine + tRNACys | SepRS differs from CysRS by recruiting the m1G37 modification as a determinant for aminoacylation, and in showing limited discrimination against mutations of conserved nucleotides. The enzyme requires the S-adenosylmethione-dependent formation of m1G37 in the anticodon loop for efficient aminoacylation | Methanocaldococcus jannaschii | AMP + diphosphate + O-phospho-L-seryl-tRNACys | - |
? | |
6.1.1.27 | additional information | Methanocaldococcus jannaschii synthesizes Cys-tRNACys by an indirect pathway, whereby O-phosphoseryl-tRNA synthetase acylates tRNACys with phosphoserine, and Sep-tRNA-Cys-tRNA synthase converts the tRNA-bound phosphoserine to cysteine. Methanocaldococcus jannaschii SepRS differs from CysRS by recruiting the m1G37 modification as a determinant for aminoacylation | Methanocaldococcus jannaschii | ? | - |
? | |
6.1.1.27 | additional information | kinetic and binding measurements show that both SepRS and Sep-tRNA-Cys-tRNA synthase, SepCysS, bind the reaction intermediate Sep-tRNACys tightly, and these two enzymes form a stable binary complex that promotes conversion of the intermediate to the product and sequesters the intermediate from binding to elongation factor EF-1alpha or infiltrating into the ribosome, mechanism of the binary complex, detailed overview | Methanocaldococcus jannaschii | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.5.1.73 | More | both SepRS and SepCysS are active as a monomer in theSepRSSepCysS binary complex | Methanocaldococcus jannaschii |
6.1.1.27 | More | both SepRS and SepCysS are active as a monomer in theSepRSSepCysS binary complex | Methanocaldococcus jannaschii |
6.1.1.27 | tetramer | SepRS contains two tRNACys molecules per tetramer indicating an asymmetry of the four identical subunits | Methanocaldococcus jannaschii |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.73 | Sep-tRNA-Cys-tRNA synthase | both SepRS and SepCysS bind the reaction intermediate Sep-tRNACys tightly, and these two enzymes form a stable binary complex that promotes conversion of the intermediate to the product and sequesters the intermediate from binding to elongation factor EF-1alpha or infiltrating into the ribosome | Methanocaldococcus jannaschii |
2.5.1.73 | SepCysS | - |
Methanocaldococcus jannaschii |
2.5.1.73 | SepCysS | SepRSSepCysS binary complex | Methanocaldococcus jannaschii |
6.1.1.16 | CysRS | - |
Escherichia coli |
6.1.1.16 | Cysteinyl-tRNA synthetase | - |
Escherichia coli |
6.1.1.27 | SepRS | SepRSSepCysS binary complex | Methanocaldococcus jannaschii |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.1.1.27 | 0.07 | - |
tRNACys | 60°C, pH 6.0, steady-state kinetics | Methanocaldococcus jannaschii | |
6.1.1.27 | 0.24 | - |
m1G37-tRNACys | 60°C, pH 6.0, steady-state kinetics | Methanocaldococcus jannaschii | |
6.1.1.27 | 0.24 | - |
tRNACys | pH 7.5, 37°C, recombinant enzyme, m1G37 tRNACys | Methanocaldococcus jannaschii | |
6.1.1.27 | 1 | - |
tRNACys | pH 7.5, 37°C, recombinant enzyme, native tRNACys | Methanocaldococcus jannaschii |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
6.1.1.27 | ATP | - |
Methanocaldococcus jannaschii |