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Literature summary extracted from

  • Seo, S.N.; Lee, J.H.; Kim, Y.M.
    Characterization of an iron- and manganese-containing superoxide dismutase from Methylobacillus sp. strain SK1 DSM 8269 (2007), Mol. Cells, 23, 370-378.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.15.1.1 H2O2 H2O2 does not show significant inhibition of the SOD activity in cell-free extracts prepared from cells grown in Mn-rich medium, but inhibits 30% of the enzyme activity in cell extracts from cells grown in Fe-rich medium Methylobacillus sp.
1.15.1.1 NaN3 inactivates 15% and 24% of the SODs present in cell extracts prepared from cells grown in the Mn- and Fe-rich media, respectively Methylobacillus sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.15.1.1 Fe2+ a Fe,Mn-SOD, the purified enzyme contains 1.1 g-atom of Fe per mol enzyme Methylobacillus sp.
1.15.1.1 Mn2+ a Fe,Mn-SOD, the purified enzyme contains 0.7 g-atom of Mn per mol enzyme Methylobacillus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.15.1.1 23100
-
 2 * 23100, SDS-PAGE Methylobacillus sp.
1.15.1.1 44000
-
 gel filtration Methylobacillus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.15.1.1 O2.- + H+ Methylobacillus sp.
-
 O2 + H2O2
-
 ?
1.15.1.1 O2.- + H+ Methylobacillus sp. SK1 / DSM 8269
-
 O2 + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.15.1.1 Methylobacillus sp.
-
-
-
1.15.1.1 Methylobacillus sp. SK1 / DSM 8269
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.15.1.1 native enzyme 61.5fold by ammonium sulfate fractionation, anion exchange chromatography, gel filtration, hydrophobic interaction chromatography and again gel filtration, to homogeneity Methylobacillus sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.15.1.1 additional information
-
 purified native enzyme Methylobacillus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.15.1.1 O2.- + H+
-
 Methylobacillus sp. O2 + H2O2
-
 ?
1.15.1.1 O2.- + H+
-
 Methylobacillus sp. SK1 / DSM 8269 O2 + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.15.1.1 dimer 2 * 23100, SDS-PAGE Methylobacillus sp.

Synonyms

EC Number Synonyms Comment Organism
1.15.1.1 Fe/MnSOD
-
 Methylobacillus sp.
1.15.1.1 iron-containing superoxide dismutase
-
 Methylobacillus sp.
1.15.1.1 manganese-containing superoxide dismutase
-
 Methylobacillus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.15.1.1 50
-
 30 min, purified enzyme, completely stable Methylobacillus sp.
1.15.1.1 60
-
 30 min, purified enzyme, 73% remaining activity Methylobacillus sp.
1.15.1.1 70
-
 10 min, purified enzyme, 46% remaining activity, inactivation after 20 min Methylobacillus sp.
1.15.1.1 80
-
 10 min, purified enzyme, 13% remaining activity, inactivation after 15 min Methylobacillus sp.
1.15.1.1 90
-
 5 min, purified enzyme, inactivation Methylobacillus sp.
1.15.1.1 100
-
 4 min, purified enzyme, inactivation Methylobacillus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.15.1.1 8
-
-
 Methylobacillus sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.15.1.1 5 10
-
 Methylobacillus sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.15.1.1 Methylobacillus sp. isoelectric focusing
-
 4.4