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Literature summary extracted from
- The disulfide redox system of Schistosoma mansoni and the importance of a multifunctional enzyme, thioredoxin glutathione reductase (2002), Mol. Biochem. Parasitol., 121, 129-139.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.8.1.B1 | expression in Escherichia coli as a six-histidine tagged protein. Recombinant His-SmTGR, which would not have a penultimate selenocysteine when expressed in Escherichia coli, has no measurable activity in the insulin reduction assay | Schistosoma mansoni |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.1.B1 | auranofin | - |
Schistosoma mansoni |  |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.1.B1 | selenium | selenocysteine protein | Schistosoma mansoni | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.1.B1 | Schistosoma mansoni | Q962Y6 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.1.B1 | recombinant enzyme | Schistosoma mansoni |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.8.1.B1 | adult | thioredoxin glutathione reductase appears to be the major, if not the sole enzyme for these activities in adult worms, completely replacing thioredoxin reductase and glutathione reductase | Schistosoma mansoni | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.1.B1 | 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+ | - |
Schistosoma mansoni | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
| 1.8.1.B1 | GSSG + NADPH + H+ | - |
Schistosoma mansoni | glutathione + NADP+ | - |
? | |
| 1.8.1.B1 | thioredoxin disulfide + NADPH + H+ | - |
Schistosoma mansoni | thioredoxin + NADP+ | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.8.1.B1 | SmTGR | - |
Schistosoma mansoni |
| 1.8.1.B1 | thioredoxin glutathione reductase | - |
Schistosoma mansoni |
IC50 Value
| EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|---|
| 1.8.1.B1 | 0.000007 | - |
pH 7.0, 25°C, thioredoxin reductase activity | Schistosoma mansoni | auranofin | |
| 1.8.1.B1 | 0.00001 | - |
pH 7.0, 37°C, glutathione reductase activity | Schistosoma mansoni | auranofin | |
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Release 2023.1 (January 2023)
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