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Literature summary extracted from

  • Kremer, A.; Westrich, L.; Li, S.M.
    A 7-dimethylallyltryptophan synthase from Aspergillus fumigatus: overproduction, purification and biochemical characterization (2007), Microbiology, 153, 3409-3416.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.80 expression in Escherichia coli, His-tagged protein Aspergillus fumigatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.80 0.067
-
 dimethylallyl diphosphate pH 7.5, 37°C Aspergillus fumigatus
2.5.1.80 0.137
-
 L-tryptophan pH 7.5, 37°C Aspergillus fumigatus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.80 Ca2+ slight stimulation Aspergillus fumigatus
2.5.1.80 additional information addition of divalent cations is not required Aspergillus fumigatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.80 53000
-
 x * 53000, calculated Aspergillus fumigatus

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.80 Aspergillus fumigatus Q4WYG3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.80 recombinant protein Aspergillus fumigatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.80 dimethylallyl diphosphate + cyclo-L-Trp-Gly
-
 Aspergillus fumigatus diphosphate + cyclo-L-7-(3-methylbut-2-enyl)-Trp-Gly 1.8% of the activity with L-tryptophan ?
2.5.1.80 dimethylallyl diphosphate + D-tryptophan
-
 Aspergillus fumigatus diphosphate + D-7-(3-methylbut-2-enyl)-tryptophan 15.5% of the activity with L-tryptophan ?
2.5.1.80 dimethylallyl diphosphate + L-Trp-Gly
-
 Aspergillus fumigatus diphosphate + L-7-(3-methylbut-2-enyl)-Trp-Gly 10.9% of the activity with L-tryptophan ?
2.5.1.80 dimethylallyl diphosphate + L-tryptophan
-
 Aspergillus fumigatus diphosphate + L-7-(3-methylbut-2-enyl)-tryptophan
-
 ?
2.5.1.80 additional information no substrate: geranyl diphosphate, L-phenylalanine, L-tyrosine Aspergillus fumigatus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.80 ? x * 53000, calculated Aspergillus fumigatus