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Literature summary extracted from

  • Vangnai, A.S.; Arp, D.J.
    An inducible 1-butanol dehydrogenase, a quinohaemoprotein, is involved in the oxidation of butane by Pseudomonas butanovora (2001), Microbiology, 147, 745-756.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.5.2 additional information the enzyme is induced by growth on 1-butanol and 2-butanol Thauera butanivorans
1.2.5.2 NH4+ 3fold activation at 4 mM Thauera butanivorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.2.9 0.007
-
 butan-1-ol at pH 8.0 and 25°C Thauera butanivorans
1.2.5.2 0.007
-
 1-butanol
-
 Thauera butanivorans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.2.9 soluble
-
 Thauera butanivorans
-
-
1.2.5.2 soluble
-
 Thauera butanivorans
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.2.9 Ca2+ calcium ions (up to 10 mM) increase the enzyme activity substantially Thauera butanivorans
1.1.2.9 NH4+ activity is increased approximately 3fold in the presence of ammonium ions (4 mM) Thauera butanivorans
1.2.5.2 Ca2+ highly activating Thauera butanivorans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.2.9 66000
-
 1 * 66000, SDS-PAGE Thauera butanivorans
1.1.2.9 69000
-
 gel filtration Thauera butanivorans
1.2.5.2 66000
-
 1 * 66000, SDS-PAGE Thauera butanivorans
1.2.5.2 69000
-
 gel filtration Thauera butanivorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.2.9 butan-1-ol + ferricytochrome c Thauera butanivorans
-
 butanal + ferrocytochrome c + H+
-
 ?
1.2.5.2 1-butanol + acceptor Thauera butanivorans the NAD+-independent inducible 1-butanol dehydrogenase, a quinohemoprotein, is responsible for 1-butanol oxidation in the butane metabolism pathway butanal + reduced acceptor
-
 ?
1.2.5.2 1-butanol + acceptor Thauera butanivorans ATCC 43655 the NAD+-independent inducible 1-butanol dehydrogenase, a quinohemoprotein, is responsible for 1-butanol oxidation in the butane metabolism pathway butanal + reduced acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.2.9 Thauera butanivorans
-
-
-
1.1.2.9 Thauera butanivorans ATCC 43655
-
-
-
1.2.5.2 Thauera butanivorans
-
 strain ATCC 43655
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.2.9 ammonium sulfate precipitation, 4-amino-butanol affinity column chromatography, and Q-Sepharose column chromatography Thauera butanivorans
1.2.5.2 native enzyme 37fold to near homogeneity by ammonium sulfate fractionation and anion exchange chromatography Thauera butanivorans

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.2.5.2 Ca2+ ions facilitate the reconstitution of inactive apoenzyme Thauera butanivorans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.2.9 culture condition:butane-grown cell
-
 Thauera butanivorans
-
1.2.5.2 culture condition:1-butanol-grown cell
-
 Thauera butanivorans
-
1.2.5.2 culture condition:2-butanol-grown cell
-
 Thauera butanivorans
-
1.2.5.2 culture condition:butane-grown cell
-
 Thauera butanivorans
-
1.2.5.2 culture condition:D-lactate-grown cell
-
 Thauera butanivorans
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.2.9 0.13
-
 crude supernatant, at pH 8.0 and 25°C Thauera butanivorans
1.1.2.9 4.8
-
 after 37fold purification, at pH 8.0 and 25°C Thauera butanivorans
1.2.5.2 0.0019
-
 lactate-grown cell extract Thauera butanivorans
1.2.5.2 0.0044
-
 2-butanol-grown cell extract Thauera butanivorans
1.2.5.2 0.0106
-
 1-butanol-grown cell extract Thauera butanivorans
1.2.5.2 4.8
-
 purified enzyme Thauera butanivorans

Storage Stability

EC Number Storage Stability Organism
1.1.2.9 -80, 25 mM Tris-HCl pH 8.0, more than 6 months, no loss of activity Thauera butanivorans
1.2.5.2 -80°C, purified enzyme, 25 mM Tris-HCl, pH 8.0, stable for more than 6 months Thauera butanivorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.2.9 1,2-butanediol + ferricytochrome c 30% activity compared to 1-butan-1-ol Thauera butanivorans ?
-
 ?
1.1.2.9 1,2-propanediol + ferricytochrome c 4% activity compared to 1-butan-1-ol Thauera butanivorans ?
-
 ?
1.1.2.9 1,3-propanediol + ferricytochrome c 5% activity compared to 1-butan-1-ol Thauera butanivorans ?
-
 ?
1.1.2.9 1,4-butanediol + ferricytochrome c 24% activity compared to 1-butan-1-ol Thauera butanivorans ?
-
 ?
1.1.2.9 1,4-pentanediol + ferricytochrome c 1% activity compared to 1-butan-1-ol Thauera butanivorans ?
-
 ?
1.1.2.9 1-heptanol + ferricytochrome c 31% activity compared to 1-butan-1-ol Thauera butanivorans heptanal + ferrocytochrome c + H+
-
 ?
1.1.2.9 1-hexanol + ferricytochrome c 29% activity compared to 1-butan-1-ol Thauera butanivorans hexanal + ferrocytochrome c + H+
-
 ?
1.1.2.9 1-nonanol + ferricytochrome c 9% activity compared to 1-butan-1-ol Thauera butanivorans nonanal + ferrocytochrome c + H+
-
 ?
1.1.2.9 1-octanol + ferricytochrome c 19% activity compared to 1-butan-1-ol Thauera butanivorans octanal + ferrocytochrome c + H+
-
 ?
1.1.2.9 1-pentanol + ferricytochrome c 45% activity compared to 1-butan-1-ol Thauera butanivorans pentanal + ferrocytochrome c + H+
-
 ?
1.1.2.9 1-propanol + ferricytochrome c 66% activity compared to 1-butan-1-ol Thauera butanivorans propanal + ferrocytochrome c + H+
-
 ?
1.1.2.9 2,3-butanediol + ferricytochrome c 2% activity compared to 1-butan-1-ol Thauera butanivorans ?
-
 ?
1.1.2.9 2,4-pentanediol + ferricytochrome c 2% activity compared to 1-butan-1-ol Thauera butanivorans ?
-
 ?
1.1.2.9 2-butanol + ferricytochrome c 9% activity compared to 1-butan-1-ol Thauera butanivorans 2-butanone + ferrocytochrome c + H+
-
 ?
1.1.2.9 2-heptanol + ferricytochrome c 49% activity compared to 1-butan-1-ol Thauera butanivorans 2-heptanone + ferrocytochrome c + H+
-
 ?
1.1.2.9 2-hexanol + ferricytochrome c 56% activity compared to 1-butan-1-ol Thauera butanivorans ?
-
 ?
1.1.2.9 2-nonanol + ferricytochrome c 35% activity compared to 1-butan-1-ol Thauera butanivorans 2-nonanone + ferrocytochrome c + H+
-
 ?
1.1.2.9 2-octanol + ferricytochrome c 42% activity compared to 1-butan-1-ol Thauera butanivorans 2-octanone + ferrocytochrome c + H+
-
 ?
1.1.2.9 2-pentanol + ferricytochrome c 68% activity compared to 1-butan-1-ol Thauera butanivorans 2-pentanone + ferrocytochrome c + H+
-
 ?
1.1.2.9 2-propanol + ferricytochrome c 52% activity compared to 1-butan-1-ol Thauera butanivorans ?
-
 ?
1.1.2.9 acetaldehyde + ferricytochrome c 54% activity compared to 1-butan-1-ol Thauera butanivorans ?
-
 ?
1.1.2.9 benzyl alcohol + ferricytochrome c 11% activity compared to 1-butan-1-ol Thauera butanivorans benzaldehyde + ferrocytochrome c + H+
-
 ?
1.1.2.9 butan-1-ol + ferricytochrome c
-
 Thauera butanivorans butanal + ferrocytochrome c + H+
-
 ?
1.1.2.9 butyraldehyde + ferricytochrome c 78% activity compared to 1-butan-1-ol Thauera butanivorans ?
-
 ?
1.1.2.9 ethanol + ferricytochrome c 34% activity compared to 1-butan-1-ol Thauera butanivorans acetaldehyde + ferrocytochrome c + H+
-
 ?
1.1.2.9 methanol + ferricytochrome c 2% activity compared to 1-butan-1-ol Thauera butanivorans formaldehyde + ferrocytochrome c + H+
-
 ?
1.1.2.9 additional information the enzyme also exhibits ferricyanide-dependent alcohol dehydrogenase activity Thauera butanivorans ?
-
 ?
1.1.2.9 phenol + ferricytochrome c 13% activity compared to 1-butan-1-ol Thauera butanivorans ?
-
 ?
1.1.2.9 propionaldehyde + ferricytochrome c 61% activity compared to 1-butan-1-ol Thauera butanivorans ?
-
 ?
1.2.5.2 1-butanol + acceptor the NAD+-independent inducible 1-butanol dehydrogenase, a quinohemoprotein, is responsible for 1-butanol oxidation in the butane metabolism pathway Thauera butanivorans butanal + reduced acceptor
-
 ?
1.2.5.2 1-butanol + acceptor the NAD+-independent inducible 1-butanol dehydrogenase, a quinohemoprotein, is responsible for 1-butanol oxidation in the butane metabolism pathway Thauera butanivorans ATCC 43655 butanal + reduced acceptor
-
 ?
1.2.5.2 acetaldehyde + 2,6-dichlorophenolindophenol
-
 Thauera butanivorans acetate + reduced 2,6-dichlorophenolindophenol
-
 ?
1.2.5.2 acetaldehyde + 2,6-dichlorophenolindophenol
-
 Thauera butanivorans ATCC 43655 acetate + reduced 2,6-dichlorophenolindophenol
-
 ?
1.2.5.2 butyraldehyde + 2,6-dichlorophenolindophenol
-
 Thauera butanivorans butanoate + reduced 2,6-dichlorophenolindophenol
-
 ?
1.2.5.2 butyraldehyde + 2,6-dichlorophenolindophenol
-
 Thauera butanivorans ATCC 43655 butanoate + reduced 2,6-dichlorophenolindophenol
-
 ?
1.2.5.2 additional information the enzyme has a broad substrate range, including primary alcohols, secondary alcohols, aldehydes, C4 diols and aromatic alcohols, BDH exhibits a marked preference towards 2-pentanol and the activity gradually decreases with longer-chain secondary alcohols, BDH exhibits ferricyanide-dependent ADH activity Thauera butanivorans ?
-
 ?
1.2.5.2 additional information the enzyme has a broad substrate range, including primary alcohols, secondary alcohols, aldehydes, C4 diols and aromatic alcohols, BDH exhibits a marked preference towards 2-pentanol and the activity gradually decreases with longer-chain secondary alcohols, BDH exhibits ferricyanide-dependent ADH activity Thauera butanivorans ATCC 43655 ?
-
 ?
1.2.5.2 propionaldehyde + 2,6-dichlorophenolindophenol
-
 Thauera butanivorans propanoate + reduced 2,6-dichlorophenolindophenol
-
 ?
1.2.5.2 propionaldehyde + 2,6-dichlorophenolindophenol
-
 Thauera butanivorans ATCC 43655 propanoate + reduced 2,6-dichlorophenolindophenol
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.2.9 monomer 1 * 66000, SDS-PAGE Thauera butanivorans
1.2.5.2 monomer 1 * 66000, SDS-PAGE Thauera butanivorans

Synonyms

EC Number Synonyms Comment Organism
1.1.2.9 1-butanol dehydrogenase
-
 Thauera butanivorans
1.1.2.9 BDH
-
 Thauera butanivorans
1.1.5.5 BDH
-
-
1.1.5.11 1-butanol dehydrogenase
-
-
1.1.5.11 NAD+-independent 1-butanol dehydrogenase inducible
-

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.2.9 60
-
-
 Thauera butanivorans
1.2.5.2 60
-
 phenazine methosulfate/2,6-dichlorophenolindophenol-dependent activity Thauera butanivorans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.2.9 25 60 the temperature range of maximum stability of the enzyme is 25-32°C, within which the loss of enzyme activity is insignificant after a 30 min incubation. In contrast, enzyme activity is decreased 77% when incubated at 60°C for the same period of time Thauera butanivorans
1.2.5.2 25 32 purified enzyme, maximally stable within this range Thauera butanivorans
1.2.5.2 60
-
 30 min, purified enzyme, loss of 77% activity Thauera butanivorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.2.9 8
-
-
 Thauera butanivorans
1.2.5.2 8
-
 phenazine methosulfate/2,6-dichlorophenolindophenol-dependent activity Thauera butanivorans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.2.9 heme c
-
 Thauera butanivorans
1.1.2.9 additional information NAD+-independent Thauera butanivorans
1.1.2.9 pyrroloquinoline quinone
-
 Thauera butanivorans
1.2.5.2 heme c 0.25 mol of heme per mol of enzyme Thauera butanivorans
1.2.5.2 pyrroloquinoline quinone PQQ, 1.0 mol of PQQ per mol of enzyme Thauera butanivorans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.2.9 720
-
 butan-1-ol at pH 8.0 and 25°C Thauera butanivorans