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Literature summary extracted from

  • Bhosale, S.H.; Pant, A.; Khan, M.I.
    Purification and characterization of putative alkaline [Ni-Fe] hydrogenase from unicellular marine green alga, Tetraselmis kochinensis NCIM 1605 (2007), Microbiol. Res., 164, 131-137.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.12.99.6 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide 8 residues modified by 0.5 mM in the presence of 30 mM nitrotyrosine ethylester Tetraselmis sp. KSN-2002
1.12.99.6 5-5'dithiobis-(2-nitrobenzoate) chemical modification of cysteine residues, 8 residues modified by 0.5 mM. 16 residues modified in the presence of 2% sodium dodecyl sulfate. 20 residues modified by 1 mM and in the presence of urea, EDTA, NaBH4 Tetraselmis sp. KSN-2002

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.7.2 Fe2+ contains 10 g atoms of Fe per mole of protein Tetraselmis sp. KSN-2002
1.12.7.2 Ni2+ contains 1 g atoms of Ni per mole of protein Tetraselmis sp. KSN-2002
1.12.99.6 Fe has Fe-S clusters, contains 10 g atoms of Fe per mol of protein Tetraselmis sp. KSN-2002
1.12.99.6 Ni contains 1 g of atom of Ni per mol of protein Tetraselmis sp. KSN-2002

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.7.2 19000
-
 1 * 19000 + 1 * 70000, SDS-PAGE Tetraselmis sp. KSN-2002
1.12.7.2 70000
-
 1 * 19000 + 1 * 70000, SDS-PAGE Tetraselmis sp. KSN-2002
1.12.7.2 89000
-
 SDS-PAGE Tetraselmis sp. KSN-2002
1.12.99.6 19000
-
 1 * 70000 + 1 * 19000, calculated Tetraselmis sp. KSN-2002
1.12.99.6 70000
-
 1 * 70000 + 1 * 19000, calculated Tetraselmis sp. KSN-2002

Organism

EC Number Organism UniProt Comment Textmining
1.12.7.2 Tetraselmis sp. KSN-2002
-
-
-
1.12.7.2 Tetraselmis sp. KSN-2002 NCIM 1605
-
-
-
1.12.99.6 Tetraselmis sp. KSN-2002
-
 NCIM 1605
-
1.12.99.6 Tetraselmis sp. KSN-2002 NCIM 1605
-
 NCIM 1605
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.99.6 467fold with a yield of 0.13%, to homogeneity, under anaerobic and reducing conditions using sodium dithionite as reducing agent, by ultrafiltration Tetraselmis sp. KSN-2002

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12.7.2 0.107
-
 crude extract Tetraselmis sp. KSN-2002
1.12.7.2 50
-
 after 467.2fold purification Tetraselmis sp. KSN-2002
1.12.99.6 0.107
-
 crude extract Tetraselmis sp. KSN-2002
1.12.99.6 50
-
 467fold purified enzyme Tetraselmis sp. KSN-2002

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.7.2 H2 + oxidized ferredoxin
-
 Tetraselmis sp. KSN-2002 H+ + reduced ferredoxin
-
 ?
1.12.7.2 H2 + oxidized ferredoxin
-
 Tetraselmis sp. KSN-2002 NCIM 1605 H+ + reduced ferredoxin
-
 ?
1.12.7.2 H2 + oxidized methyl viologen
-
 Tetraselmis sp. KSN-2002 H+ + reduced methyl viologen
-
 ?
1.12.7.2 H2 + oxidized methyl viologen
-
 Tetraselmis sp. KSN-2002 NCIM 1605 H+ + reduced methyl viologen
-
 ?
1.12.99.6 H2 + oxidized methyl viologen
-
 Tetraselmis sp. KSN-2002 H+ + reduced methyl viologen
-
 ?
1.12.99.6 H2 + oxidized methyl viologen
-
 Tetraselmis sp. KSN-2002 NCIM 1605 H+ + reduced methyl viologen
-
 ?

Subunits

EC Number Subunits Comment Organism
1.12.7.2 heterodimer 1 * 19000 + 1 * 70000, SDS-PAGE Tetraselmis sp. KSN-2002
1.12.99.6 dimer 1 * 70000 + 1 * 19000, calculated Tetraselmis sp. KSN-2002

Synonyms

EC Number Synonyms Comment Organism
1.12.7.2 [Ni-Fe] hydrogenase
-
 Tetraselmis sp. KSN-2002
1.12.99.6 [Ni-Fe] hydrogenase
-
 Tetraselmis sp. KSN-2002

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.12.7.2 45
-
-
 Tetraselmis sp. KSN-2002
1.12.99.6 45
-
-
 Tetraselmis sp. KSN-2002

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.12.7.2 9
-
-
 Tetraselmis sp. KSN-2002
1.12.99.6 9
-
-
 Tetraselmis sp. KSN-2002

Cofactor

EC Number Cofactor Comment Organism Structure
1.12.7.2 iron-sulfur centre
-
 Tetraselmis sp. KSN-2002

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.12.7.2 Tetraselmis sp. KSN-2002 isoelectric focusing
-
 9.6
1.12.99.6 Tetraselmis sp. KSN-2002 isoelectric focusing
-
 9.6