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Literature summary extracted from

  • Hong, Y.; Wang, G.; Maier, R.J.
    The NADPH quinone reductase MdaB confers oxidative stress resistance to Helicobacter hepaticus (2008), Microb. Pathog., 44, 169-174.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.6.5.2 into pET21a, His-tagged version overexpressed in Escherichia coli BL21 Origami competent cells Helicobacter hepaticus

Protein Variants

EC Number Protein Variants Comment Organism
1.6.5.2 additional information mdaB mutant can be grown in microaerophilic conditions. The mutant is more sensitive to oxidative stress reagents such as H2O2, cumene hydroperoxide, t-butyl hydroperoxide, and paraquat. Protein (migration mass of about 26 kDa) is significantly upexpressed in the mutant compared to the wild-type Helicobacter hepaticus

Organism

EC Number Organism UniProt Comment Textmining
1.6.5.2 Helicobacter hepaticus
-
 strain ATCC51449
-
1.6.5.2 Helicobacter hepaticus ATCC 51449
-
 strain ATCC51449
-
1.6.5.10 Helicobacter hepaticus
-
 strain ATCC51449
-
1.6.5.10 Helicobacter hepaticus ATCC 51449
-
 strain ATCC51449
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.5.10
-
 Helicobacter hepaticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.5.2 additional information
-
 quinone or quinone-like compounds reduction assay with a specific activity ranging from 0.92-4.6 U/mg of protein Helicobacter hepaticus
1.6.5.2 4
-
 menadione-cytochrome c reduction assay Helicobacter hepaticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.5.2 1,4-naphthoquinone + NAD(P)H
-
 Helicobacter hepaticus 1,4-naphthohydroquinone + NAD(P)+
-
 ?
1.6.5.2 1,4-naphthoquinone + NAD(P)H
-
 Helicobacter hepaticus ATCC 51449 1,4-naphthohydroquinone + NAD(P)+
-
 ?
1.6.5.2 2 ferricyanide + NAD(P)H
-
 Helicobacter hepaticus 2 ferrocyanide + NAD(P)+ + H+
-
 ?
1.6.5.2 2 ferricyanide + NAD(P)H
-
 Helicobacter hepaticus ATCC 51449 2 ferrocyanide + NAD(P)+ + H+
-
 ?
1.6.5.2 coenzyme Q0 + NAD(P)H
-
 Helicobacter hepaticus reduced coenzyme Q0 + NADP+
-
 ?
1.6.5.2 coenzyme Q0 + NAD(P)H
-
 Helicobacter hepaticus ATCC 51449 reduced coenzyme Q0 + NADP+
-
 ?
1.6.5.2 coenzyme Q1 + NAD(P)H
-
 Helicobacter hepaticus reduced coenzyme Q1 + NADP+
-
 ?
1.6.5.2 coenzyme Q1 + NAD(P)H
-
 Helicobacter hepaticus ATCC 51449 reduced coenzyme Q1 + NADP+
-
 ?
1.6.5.2 menadione + NAD(P)H
-
 Helicobacter hepaticus reduced menadione + NADP+
-
 ?
1.6.5.2 NAD(P)H + H+ + quinone
-
 Helicobacter hepaticus NAD(P)+ + hydroquinone
-
 ?
1.6.5.2 oxidized dichlorophenol-indophenol + NAD(P)H
-
 Helicobacter hepaticus reduced dichlorophenol-indophenol + NADP+
-
 ?
1.6.5.2 oxidized dichlorophenol-indophenol + NAD(P)H
-
 Helicobacter hepaticus ATCC 51449 reduced dichlorophenol-indophenol + NADP+
-
 ?
1.6.5.10 1,4-naphthoquinone + NADPH + H+ two-electron transfer mechanism from NADPH to quinone Helicobacter hepaticus 1,4-naphthoquinol + NADP+
-
 ?
1.6.5.10 2 ferricyanide + NADPH
-
 Helicobacter hepaticus 2 ferrocyanide + NADP+ + H+
-
 ?
1.6.5.10 menadione + NADPH + H+ two-electron transfer mechanism from NADPH to quinone Helicobacter hepaticus ? + NADP+
-
 ?
1.6.5.10 oxidized dichlorophenolindophenol + NADPH + H+
-
 Helicobacter hepaticus reduced dicholorophenolindophenol + NADP+
-
 ?
1.6.5.10 ubiquinone-0 + NADPH + H+ two-electron transfer mechanism from NADPH to quinone Helicobacter hepaticus ubiquinol-0 + NADP+
-
 ?
1.6.5.10 ubiquinone-1 + NADPH + H+ two-electron transfer mechanism from NADPH to quinone Helicobacter hepaticus ubiquinol-1 + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.6.5.2 ? x * 21-22000, SDS-PAGE Helicobacter hepaticus
1.6.5.10 ? x * 21000-22000, SDS-PAGE Helicobacter hepaticus

Synonyms

EC Number Synonyms Comment Organism
1.6.5.2 MdaB
-
 Helicobacter hepaticus
1.6.5.2 NADPH quinone reductase
-
 Helicobacter hepaticus
1.6.5.10 MdaB
-
 Helicobacter hepaticus
1.6.5.10 NADPH quinone reductase
-
 Helicobacter hepaticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.6.5.10 22
-
 assay at room temperature Helicobacter hepaticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.6.5.10 7.5
-
 assay at Helicobacter hepaticus

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.5.2 NADH
-
 Helicobacter hepaticus
1.6.5.2 NADPH
-
 Helicobacter hepaticus
1.6.5.10 flavin flavoprotein, adsorption spectrum with a major peak at 456 nm Helicobacter hepaticus
1.6.5.10 NADPH NADH could also be used as an electron donor to MdaB but with much lower efficiency, about 3% of those with NADPH Helicobacter hepaticus