Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Cho, Y.; Lee, H.S.; Kim, Y.J.; Kang, S.G.; Kim, S.J.; Lee, J.H.
    Characterization of a dUTPase from the hyperthermophilic archaeon Thermococcus onnurineus NA1 and its application in polymerase chain reaction amplification (2007), Mar. Biotechnol., 9, 450-458.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.23 DNA and amino acid sequence determination and analysis, His-tagged enzyme expression in Escherichia coli Thermococcus onnurineus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.23 bovine serum albumin 38% inhibition at 0.01% Thermococcus onnurineus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.23 additional information the enzymatic activity of the dUTPase is largely unaffected by variations in MgCl2, KCl, (NH4)2SO4, and Triton X-100 concentrations Thermococcus onnurineus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.23 17700
-
 2 * 17700, about, sequence calculation Thermococcus onnurineus
3.6.1.23 43000
-
 recombinant enzyme, gel filtration Thermococcus onnurineus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.23 dCTP + H2O Thermococcus onnurineus
-
 dCMP + diphosphate
-
 ?
3.6.1.23 dUTP + H2O Thermococcus onnurineus the enzyme plays an essential role in nucleotide biosynthesis, dUMP is required for de novo synthesis of dTTP. The enzyme maintains low cellular ratios of dUTP:dTTP, thus preventing the misincorporation of uracil into chromosomal DNA dUMP + diphosphate
-
 ?
3.6.1.23 additional information Thermococcus onnurineus the enzyme is essential for survival ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.23 Thermococcus onnurineus A7J1X3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.23 recombinant His-tagged enzyme from Escherichia coli by cobalt chelating affinity chromatography and gel filtration Thermococcus onnurineus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.23 dCTP + H2O
-
 Thermococcus onnurineus dCMP + diphosphate
-
 ?
3.6.1.23 dCTP + H2O about 10fold lower activity with dCTP compared to dUTP Thermococcus onnurineus dCMP + diphosphate
-
 ?
3.6.1.23 dUTP + H2O preferred substrate Thermococcus onnurineus dUMP + diphosphate
-
 ?
3.6.1.23 dUTP + H2O the enzyme plays an essential role in nucleotide biosynthesis, dUMP is required for de novo synthesis of dTTP. The enzyme maintains low cellular ratios of dUTP:dTTP, thus preventing the misincorporation of uracil into chromosomal DNA Thermococcus onnurineus dUMP + diphosphate
-
 ?
3.6.1.23 additional information the enzyme is essential for survival Thermococcus onnurineus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.23 dimer 2 * 17700, about, sequence calculation Thermococcus onnurineus

Synonyms

EC Number Synonyms Comment Organism
3.6.1.23 deoxyuridine-triphosphate pyrophosphatase
-
 Thermococcus onnurineus
3.6.1.23 dUTPase
-
 Thermococcus onnurineus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.23 80
-
-
 Thermococcus onnurineus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.6.1.23 70 95 temperature profile Thermococcus onnurineus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.6.1.23 95
-
 purified recombinant enzyme, half-life is 170 min Thermococcus onnurineus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.23 8
-
-
 Thermococcus onnurineus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.6.1.23 7.5 8.8 pH profile Thermococcus onnurineus