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Literature summary extracted from

  • Higuchi, Y.; Tanii, H.; Koriyama, Y.; Mizukami, Y.; Yoshimoto, T.
    Arachidonic acid promotes glutamate-induced cell death associated with necrosis by 12-lipoxygenase activation in glioma cells (2007), Life Sci., 80, 1856-1864.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.11.31 additional information a significant increase of 12-lipoxygenase enzyme activity in presence of arachidonic acid under GSH depletion induced by glutamate in C6 glimoa cells, overview Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.31 baicalein
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.13.11.31 membrane
-
 Rattus norvegicus 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.31 arachidonate + O2 Rattus norvegicus arachidonic acid promotes the glutamate-induced cell death, reduces caspase-3 activity, and diminishes internucleosomal DNA fragmentation, it also reduces intracellular NAD, ATP and membrane potentials due to mitochondrila membrane dysfunction, and reduces the inhibitory effect of N-alpha-tosyl-L-phenylalanine chloromethyl ketone and 3,4-dichloroisocumarin on the glutamate-induced cell death, overview (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate is further metabolized to 12(S)-hydroxyeicosatetraenoic acid ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.31 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.31 C6 glioma cell
-
 Rattus norvegicus
-
1.13.11.31 glioma cell
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.31 arachidonate + O2
-
 Rattus norvegicus (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate
-
 ?
1.13.11.31 arachidonate + O2 arachidonic acid promotes the glutamate-induced cell death, reduces caspase-3 activity, and diminishes internucleosomal DNA fragmentation, it also reduces intracellular NAD, ATP and membrane potentials due to mitochondrila membrane dysfunction, and reduces the inhibitory effect of N-alpha-tosyl-L-phenylalanine chloromethyl ketone and 3,4-dichloroisocumarin on the glutamate-induced cell death, overview Rattus norvegicus (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate is further metabolized to 12(S)-hydroxyeicosatetraenoic acid ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.31 12-lipoxygenase
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.31 30
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.31 7.4
-
 assay at Rattus norvegicus