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Literature summary extracted from

  • Li, Y.; Lu, F.; Sun, T.; Du, L.
    Expression of ksdD gene encoding 3-ketosteroid-DELTA1-dehydrogenase from Arthrobacter simplex in Bacillus subtilis (2007), Lett. Appl. Microbiol., 44, 563-568.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.99.4 expressed in Bacillus subtilis strain WB600 Pimelobacter simplex

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.99.4 55000
-
 SDS-PAGE Pimelobacter simplex

Organism

EC Number Organism UniProt Comment Textmining
1.3.99.4 Pimelobacter simplex
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.99.4 0.000015
-
 intracellular, soluble, recombinant enzyme, in 0.86 M Tris-HCl (pH9.0), 150 mM phenazine methosulfate, at 25°C, using 4-androstene-3,17-dione as substrate Pimelobacter simplex
1.3.99.4 0.000111
-
 exracellular, soluble, recombinant enzyme, in 0.86 M Tris-HCl (pH9.0), 150 mM phenazine methosulfate, at 25°C, using 4-androstene-3,17-dione as substrate Pimelobacter simplex

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.99.4 4-androstene-3,17-dione + phenazine methosulfate
-
 Pimelobacter simplex 1,4-androstene-3,17-dione + reduced phenazine methosulfate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.99.4 3-ketosteroid-DELTA1-dehydrogenase
-
 Pimelobacter simplex
1.3.99.4 KSDH
-
 Pimelobacter simplex