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Literature summary extracted from

  • Nie, Y.; Xu, Y.; Yang, M.; Mu, X.Q.
    A novel NADH-dependent carbonyl reductase with unusual stereoselectivity for (R)-specific reduction from an (S)-1-phenyl-1,2-ethanediol-producing micro-organism: purification and characterization (2007), Lett. Appl. Microbiol., 44, 555-562.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.B4 CaCl2 1 mM, 21% residual activity Candida parapsilosis
1.1.1.B4 CoCl2 1 mM, 57% residual activity Candida parapsilosis
1.1.1.B4 CuSO4 1 mM, complete loss of activity Candida parapsilosis
1.1.1.B4 EDTA 1 mM, 68% residual activity Candida parapsilosis
1.1.1.B4 FeCl3 1 mM, 65% residual activity Candida parapsilosis
1.1.1.B4 FeSO4 1 mM, complete loss of activity Candida parapsilosis
1.1.1.B4 MgSO4 1 mM, 94% residual activity Candida parapsilosis
1.1.1.B4 MnSO4 1 mM, 69% residual activity Candida parapsilosis
1.1.1.B4 NiCl2 1 mM, 96% residual activity Candida parapsilosis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.B4 soluble
-
 Candida parapsilosis
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.B4 ZnSO4 1mM, 135% of initial activity Candida parapsilosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.B4 35000
-
 gel filtration Candida parapsilosis
1.1.1.B4 37500
-
 x * 37500, SDS-PAGE Candida parapsilosis

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.B4 Candida parapsilosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.B4
-
 Candida parapsilosis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.B4 14
-
 pH 6.0, 30°C Candida parapsilosis

Storage Stability

EC Number Storage Stability Organism
1.1.1.B4 4°C, pH 6.0-8.0, 24 h, more than 90% residual activity Candida parapsilosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.B4 2-butanone + NAD+ 274% of the activity with beta-hydroxyacetophenone Candida parapsilosis 2-butanol + NADH + H+
-
 ?
1.1.1.B4 2-heptanone + NAD+ 281% of the activity with beta-hydroxyacetophenone Candida parapsilosis 2-heptanol + NADH + H+
-
 ?
1.1.1.B4 2-hexanone + NAD+ 259% of the activity with beta-hydroxyacetophenone Candida parapsilosis 2-hexanol + NADH + H+
-
 ?
1.1.1.B4 2-octanone + NAD+ 350% of the activity with beta-hydroxyacetophenone Candida parapsilosis 2-octanol + NADH + H+
-
 ?
1.1.1.B4 2-pentanone + NAD+ 249% of the activity with beta-hydroxyacetophenone Candida parapsilosis 2-pentanol + NADH + H+
-
 ?
1.1.1.B4 3-hexanone + NAD+ 29% of the activity with beta-hydroxyacetophenone Candida parapsilosis 3-hexanol + NADH + H+
-
 ?
1.1.1.B4 3-pentanone + NAD+ 35% of the activity with beta-hydroxyacetophenone Candida parapsilosis 3-pentanol + NADH + H+
-
 ?
1.1.1.B4 acetone + NAD+ 260% of the activity with beta-hydroxyacetophenone Candida parapsilosis isopropanol + NADH + H+
-
 ?
1.1.1.B4 acetophenone + NADH + H+ 171% of the activity with beta-hydroxyacetophenone Candida parapsilosis phenylethanol + NAD+
-
 ?
1.1.1.B4 beta-hydroxyacetophenone + NAD+
-
 Candida parapsilosis (R)-1-phenyl-1,2-ethanediol + NADH + H+ formation of (R)-enantiomner with 100% enantiomeric excess ?

Subunits

EC Number Subunits Comment Organism
1.1.1.B4 monomer x * 37500, SDS-PAGE Candida parapsilosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.B4 45
-
-
 Candida parapsilosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.B4 6
-
-
 Candida parapsilosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.B4 NAD+
-
 Candida parapsilosis
1.1.1.B4 NADH
-
 Candida parapsilosis