BRENDA - Enzyme Database

Chloroviruses encode a bifunctional dCMP-dCTP deaminase that produces two key intermediates in dTTP formation

Zhang, Y.; Maley, F.; Maley, G.F.; Duncan, G.; Dunigan, D.D.; Van Etten, J.L.; J. Virol. 81, 7662-7671 (2007)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.12
dCTP
activates dCMP deamination about 7fold at 0.005-0.1 mM
Paramecium bursaria Chlorella virus 1
3.5.4.13
dCTP
activates dCMP deamination about 7fold at 0.005-0.1 mM
Paramecium bursaria Chlorella virus 1
Cloned(Commentary)
EC Number
Commentary
Organism
3.5.4.12
ORF A596R, DNA and amino acid sequence determination and analysis, expression of the His6-tagged enzyme in Escherichia coli strain DH5MCR, phylogenetic analysis
Paramecium bursaria Chlorella virus 1
3.5.4.13
ORF A596R, DNA and amino acid sequence determination and analysis, expression of the His6-tagged enzyme in Escherichia coli strain DH5MCR, phylogenetic analysis
Paramecium bursaria Chlorella virus 1
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.12
dTTP
inhibits dCMP deamination, feedback inhibition
Paramecium bursaria Chlorella virus 1
3.5.4.12
H4dUMP
inhibits both dCTP and dCMP deaminase activities
Paramecium bursaria Chlorella virus 1
3.5.4.12
PDRP
inhibits both dCTP and dCMP deaminase activities
Paramecium bursaria Chlorella virus 1
3.5.4.13
dTTP
inhibits dCMP deamination, feedback inhibition
Paramecium bursaria Chlorella virus 1
3.5.4.13
H4dUMP
inhibits both dCTP and dCMP deaminase activities
Paramecium bursaria Chlorella virus 1
3.5.4.13
PDRP
inhibits both dCTP and dCMP deaminase activities
Paramecium bursaria Chlorella virus 1
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.12
0.64
-
dCTP
pH 9.5, 42C
Paramecium bursaria Chlorella virus 1
3.5.4.12
0.76
-
dCMP
pH 9.5, 42C, in presence of 0.1 mM dCTP
Paramecium bursaria Chlorella virus 1
3.5.4.13
0.64
-
dCTP
pH 9.5, 42C
Paramecium bursaria Chlorella virus 1
3.5.4.13
0.76
-
dCMP
pH 9.5, 42C, in presence of 0.1 mM dCTP
Paramecium bursaria Chlorella virus 1
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.4.12
Ca2+
can partially substitute for Mg2+
Paramecium bursaria Chlorella virus 1
3.5.4.12
Mg2+
the enzyme depends on divalent cations, Mg2+ is preferred, but can partially be substituted by Ca2+, Ni2+, or Mn2+
Paramecium bursaria Chlorella virus 1
3.5.4.12
Mn2+
can partially substitute for Mg2+
Paramecium bursaria Chlorella virus 1
3.5.4.12
Ni2+
can partially substitute for Mg2+
Paramecium bursaria Chlorella virus 1
3.5.4.12
Zn2+
the enzyme contains a zinc binding site, but shows less than 6% of maximal activity with Zn2+ as divalent cation
Paramecium bursaria Chlorella virus 1
3.5.4.13
Ca2+
can partially substitute for Mg2+
Paramecium bursaria Chlorella virus 1
3.5.4.13
Mg2+
the enzyme depends on divalent cations, Mg2+ is preferred , but can partially be substituted by Ca2+, Ni2+, or Mn2+
Paramecium bursaria Chlorella virus 1
3.5.4.13
Mn2+
can partially substitute for Mg2+
Paramecium bursaria Chlorella virus 1
3.5.4.13
Ni2+
can partially substitute for Mg2+
Paramecium bursaria Chlorella virus 1
3.5.4.13
Zn2+
the enzyme contains a zinc binding site, but shows less than 6% of maximal activity with Zn2+ as divalent cation
Paramecium bursaria Chlorella virus 1
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.12
17000
-
6 * 17000, SDS-PAGE
Paramecium bursaria Chlorella virus 1
3.5.4.13
17000
-
6 * 17000, SDS-PAGE
Paramecium bursaria Chlorella virus 1
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.12
dCMP + H2O
Paramecium bursaria Chlorella virus 1
-
dUMP + NH3
dUMP is a key intermediate in the synthesis of dTTP and subsequently of DNA synthesis
-
?
3.5.4.12
dCTP + H2O
Paramecium bursaria Chlorella virus 1
-
dUTP + NH3
-
-
?
3.5.4.13
dCMP + H2O
Paramecium bursaria Chlorella virus 1
-
dUMP + NH3
dUMP is a key intermediate in the synthesis of dTTP and subsequently of DNA synthesis
-
?
3.5.4.13
dCTP + H2O
Paramecium bursaria Chlorella virus 1
-
dUTP + NH3
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.4.12
Paramecium bursaria Chlorella virus 1
O41078
i.e. PBCV-1 , a chlorovirus from host Chlorella sp. strain NC64A
-
3.5.4.13
Paramecium bursaria Chlorella virus 1
O41078
i.e. PBCV-1 , a chlorovirus from host Chlorella sp. strain NC64A
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.4.12
recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography, the protein quickly precipitates at concentration higher than 2 mg/ml
Paramecium bursaria Chlorella virus 1
3.5.4.13
recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography, the protein quickly precipitates at concentration higher than 2 mg/ml
Paramecium bursaria Chlorella virus 1
Storage Stability
EC Number
Storage Stability
Organism
3.5.4.12
the purified recombinant His-tagged enzyme in stable without precipitation for more than 1 year in 50% glycerol
Paramecium bursaria Chlorella virus 1
3.5.4.13
the purified recombinant His-tagged enzyme in stable without precipitation for more than 1 year in 50% glycerol
Paramecium bursaria Chlorella virus 1
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.12
dCMP + H2O
-
688732
Paramecium bursaria Chlorella virus 1
dUMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
-
688732
Paramecium bursaria Chlorella virus 1
dUMP + NH3
dUMP is a key intermediate in the synthesis of dTTP and subsequently of DNA synthesis
-
-
?
3.5.4.12
dCTP + H2O
-
688732
Paramecium bursaria Chlorella virus 1
dUTP + NH3
-
-
-
?
3.5.4.12
additional information
the bifunctional dCMP-dCTP deaminase shows dCMP deaminase and dCTP deaminase activities, dCTP serves as a positive heterotropic effector for the dCMP deaminase activity and a positive homotropic effector for the dCTP deaminase activity, and the enzymatic efficiency of the dCMP deaminase activity is about four times higher than that of the dCTP deaminase activity, the same active site is involved in both dCMP and dCTP deaminations
688732
Paramecium bursaria Chlorella virus 1
?
-
-
-
-
3.5.4.13
dCMP + H2O
-
688732
Paramecium bursaria Chlorella virus 1
dUMP + NH3
-
-
-
?
3.5.4.13
dCMP + H2O
-
688732
Paramecium bursaria Chlorella virus 1
dUMP + NH3
dUMP is a key intermediate in the synthesis of dTTP and subsequently of DNA synthesis
-
-
?
3.5.4.13
dCTP + H2O
-
688732
Paramecium bursaria Chlorella virus 1
dUTP + NH3
-
-
-
?
3.5.4.13
additional information
the bifunctional dCMP-dCTP deaminase shows dCMP deaminase and dCTP deaminase activities, dCTP serves as a positive heterotropic effector for the dCMP deaminase activity and a positive homotropic effector for the dCTP deaminase activity, and the enzymatic efficiency of the dCMP deaminase activity is about four times higher than that of the dCTP deaminase activity, the same active site is involved in both dCMP and dCTP deaminations
688732
Paramecium bursaria Chlorella virus 1
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
3.5.4.12
hexamer
6 * 17000, SDS-PAGE
Paramecium bursaria Chlorella virus 1
3.5.4.13
hexamer
6 * 17000, SDS-PAGE
Paramecium bursaria Chlorella virus 1
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.5.4.12
42
-
-
Paramecium bursaria Chlorella virus 1
3.5.4.13
42
-
-
Paramecium bursaria Chlorella virus 1
Temperature Range [C]
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
3.5.4.12
25
55
-
Paramecium bursaria Chlorella virus 1
3.5.4.13
25
55
-
Paramecium bursaria Chlorella virus 1
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.5.4.12
5.7
-
dCTP
pH 9.5, 42C
Paramecium bursaria Chlorella virus 1
3.5.4.12
24.9
-
dCMP
pH 9.5, 42C, in presence of 0.1 mM dCTP
Paramecium bursaria Chlorella virus 1
3.5.4.13
5.7
-
dCTP
pH 9.5, 42C
Paramecium bursaria Chlorella virus 1
3.5.4.13
24.9
-
dCMP
pH 9.5, 42C, in presence of 0.1 mM dCTP
Paramecium bursaria Chlorella virus 1
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.4.12
7
-
dCTP deaminase activity
Paramecium bursaria Chlorella virus 1
3.5.4.12
9.5
-
dCMP deaminase activity
Paramecium bursaria Chlorella virus 1
3.5.4.13
7
-
dCTP deaminase activity
Paramecium bursaria Chlorella virus 1
3.5.4.13
9.5
-
dCMP deaminase activity
Paramecium bursaria Chlorella virus 1
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.4.12
6
11
activity range
Paramecium bursaria Chlorella virus 1
3.5.4.13
6
11
activity range
Paramecium bursaria Chlorella virus 1
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.4.12
dCTP
activates dCMP deamination about 7fold at 0.005-0.1 mM
Paramecium bursaria Chlorella virus 1
3.5.4.13
dCTP
activates dCMP deamination about 7fold at 0.005-0.1 mM
Paramecium bursaria Chlorella virus 1
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.4.12
ORF A596R, DNA and amino acid sequence determination and analysis, expression of the His6-tagged enzyme in Escherichia coli strain DH5MCR, phylogenetic analysis
Paramecium bursaria Chlorella virus 1
3.5.4.13
ORF A596R, DNA and amino acid sequence determination and analysis, expression of the His6-tagged enzyme in Escherichia coli strain DH5MCR, phylogenetic analysis
Paramecium bursaria Chlorella virus 1
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.12
dTTP
inhibits dCMP deamination, feedback inhibition
Paramecium bursaria Chlorella virus 1
3.5.4.12
H4dUMP
inhibits both dCTP and dCMP deaminase activities
Paramecium bursaria Chlorella virus 1
3.5.4.12
PDRP
inhibits both dCTP and dCMP deaminase activities
Paramecium bursaria Chlorella virus 1
3.5.4.13
dTTP
inhibits dCMP deamination, feedback inhibition
Paramecium bursaria Chlorella virus 1
3.5.4.13
H4dUMP
inhibits both dCTP and dCMP deaminase activities
Paramecium bursaria Chlorella virus 1
3.5.4.13
PDRP
inhibits both dCTP and dCMP deaminase activities
Paramecium bursaria Chlorella virus 1
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.12
0.64
-
dCTP
pH 9.5, 42C
Paramecium bursaria Chlorella virus 1
3.5.4.12
0.76
-
dCMP
pH 9.5, 42C, in presence of 0.1 mM dCTP
Paramecium bursaria Chlorella virus 1
3.5.4.13
0.64
-
dCTP
pH 9.5, 42C
Paramecium bursaria Chlorella virus 1
3.5.4.13
0.76
-
dCMP
pH 9.5, 42C, in presence of 0.1 mM dCTP
Paramecium bursaria Chlorella virus 1
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.4.12
Ca2+
can partially substitute for Mg2+
Paramecium bursaria Chlorella virus 1
3.5.4.12
Mg2+
the enzyme depends on divalent cations, Mg2+ is preferred, but can partially be substituted by Ca2+, Ni2+, or Mn2+
Paramecium bursaria Chlorella virus 1
3.5.4.12
Mn2+
can partially substitute for Mg2+
Paramecium bursaria Chlorella virus 1
3.5.4.12
Ni2+
can partially substitute for Mg2+
Paramecium bursaria Chlorella virus 1
3.5.4.12
Zn2+
the enzyme contains a zinc binding site, but shows less than 6% of maximal activity with Zn2+ as divalent cation
Paramecium bursaria Chlorella virus 1
3.5.4.13
Ca2+
can partially substitute for Mg2+
Paramecium bursaria Chlorella virus 1
3.5.4.13
Mg2+
the enzyme depends on divalent cations, Mg2+ is preferred , but can partially be substituted by Ca2+, Ni2+, or Mn2+
Paramecium bursaria Chlorella virus 1
3.5.4.13
Mn2+
can partially substitute for Mg2+
Paramecium bursaria Chlorella virus 1
3.5.4.13
Ni2+
can partially substitute for Mg2+
Paramecium bursaria Chlorella virus 1
3.5.4.13
Zn2+
the enzyme contains a zinc binding site, but shows less than 6% of maximal activity with Zn2+ as divalent cation
Paramecium bursaria Chlorella virus 1
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.12
17000
-
6 * 17000, SDS-PAGE
Paramecium bursaria Chlorella virus 1
3.5.4.13
17000
-
6 * 17000, SDS-PAGE
Paramecium bursaria Chlorella virus 1
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.12
dCMP + H2O
Paramecium bursaria Chlorella virus 1
-
dUMP + NH3
dUMP is a key intermediate in the synthesis of dTTP and subsequently of DNA synthesis
-
?
3.5.4.12
dCTP + H2O
Paramecium bursaria Chlorella virus 1
-
dUTP + NH3
-
-
?
3.5.4.13
dCMP + H2O
Paramecium bursaria Chlorella virus 1
-
dUMP + NH3
dUMP is a key intermediate in the synthesis of dTTP and subsequently of DNA synthesis
-
?
3.5.4.13
dCTP + H2O
Paramecium bursaria Chlorella virus 1
-
dUTP + NH3
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.4.12
recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography, the protein quickly precipitates at concentration higher than 2 mg/ml
Paramecium bursaria Chlorella virus 1
3.5.4.13
recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography, the protein quickly precipitates at concentration higher than 2 mg/ml
Paramecium bursaria Chlorella virus 1
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.5.4.12
the purified recombinant His-tagged enzyme in stable without precipitation for more than 1 year in 50% glycerol
Paramecium bursaria Chlorella virus 1
3.5.4.13
the purified recombinant His-tagged enzyme in stable without precipitation for more than 1 year in 50% glycerol
Paramecium bursaria Chlorella virus 1
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.12
dCMP + H2O
-
688732
Paramecium bursaria Chlorella virus 1
dUMP + NH3
-
-
-
?
3.5.4.12
dCMP + H2O
-
688732
Paramecium bursaria Chlorella virus 1
dUMP + NH3
dUMP is a key intermediate in the synthesis of dTTP and subsequently of DNA synthesis
-
-
?
3.5.4.12
dCTP + H2O
-
688732
Paramecium bursaria Chlorella virus 1
dUTP + NH3
-
-
-
?
3.5.4.12
additional information
the bifunctional dCMP-dCTP deaminase shows dCMP deaminase and dCTP deaminase activities, dCTP serves as a positive heterotropic effector for the dCMP deaminase activity and a positive homotropic effector for the dCTP deaminase activity, and the enzymatic efficiency of the dCMP deaminase activity is about four times higher than that of the dCTP deaminase activity, the same active site is involved in both dCMP and dCTP deaminations
688732
Paramecium bursaria Chlorella virus 1
?
-
-
-
-
3.5.4.13
dCMP + H2O
-
688732
Paramecium bursaria Chlorella virus 1
dUMP + NH3
-
-
-
?
3.5.4.13
dCMP + H2O
-
688732
Paramecium bursaria Chlorella virus 1
dUMP + NH3
dUMP is a key intermediate in the synthesis of dTTP and subsequently of DNA synthesis
-
-
?
3.5.4.13
dCTP + H2O
-
688732
Paramecium bursaria Chlorella virus 1
dUTP + NH3
-
-
-
?
3.5.4.13
additional information
the bifunctional dCMP-dCTP deaminase shows dCMP deaminase and dCTP deaminase activities, dCTP serves as a positive heterotropic effector for the dCMP deaminase activity and a positive homotropic effector for the dCTP deaminase activity, and the enzymatic efficiency of the dCMP deaminase activity is about four times higher than that of the dCTP deaminase activity, the same active site is involved in both dCMP and dCTP deaminations
688732
Paramecium bursaria Chlorella virus 1
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.4.12
hexamer
6 * 17000, SDS-PAGE
Paramecium bursaria Chlorella virus 1
3.5.4.13
hexamer
6 * 17000, SDS-PAGE
Paramecium bursaria Chlorella virus 1
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.5.4.12
42
-
-
Paramecium bursaria Chlorella virus 1
3.5.4.13
42
-
-
Paramecium bursaria Chlorella virus 1
Temperature Range [C] (protein specific)
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
3.5.4.12
25
55
-
Paramecium bursaria Chlorella virus 1
3.5.4.13
25
55
-
Paramecium bursaria Chlorella virus 1
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.5.4.12
5.7
-
dCTP
pH 9.5, 42C
Paramecium bursaria Chlorella virus 1
3.5.4.12
24.9
-
dCMP
pH 9.5, 42C, in presence of 0.1 mM dCTP
Paramecium bursaria Chlorella virus 1
3.5.4.13
5.7
-
dCTP
pH 9.5, 42C
Paramecium bursaria Chlorella virus 1
3.5.4.13
24.9
-
dCMP
pH 9.5, 42C, in presence of 0.1 mM dCTP
Paramecium bursaria Chlorella virus 1
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.4.12
7
-
dCTP deaminase activity
Paramecium bursaria Chlorella virus 1
3.5.4.12
9.5
-
dCMP deaminase activity
Paramecium bursaria Chlorella virus 1
3.5.4.13
7
-
dCTP deaminase activity
Paramecium bursaria Chlorella virus 1
3.5.4.13
9.5
-
dCMP deaminase activity
Paramecium bursaria Chlorella virus 1
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.4.12
6
11
activity range
Paramecium bursaria Chlorella virus 1
3.5.4.13
6
11
activity range
Paramecium bursaria Chlorella virus 1