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Literature summary extracted from

  • Meek, L.; Martin, R.C.; Shan, X.; Karplus, P.A.; Mok, D.W.; Mok, M.C.
    Isolation of Legume Glycosyltransferases and Active Site Mapping of the Phaseolus lunatus Zeatin O-glucosyltransferase ZOG1 (2008), J. Plant Growth Regul., 27, 192-201.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.203 expression in Escherichia coli Phaseolus lunatus
2.4.1.203 expression in Escherichia coli Phaseolus vulgaris
2.4.1.203 expression in Escherichia coli for sequencing Glycine max

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.203 CTC151SFS site-directed mutagenesis Phaseolus vulgaris
2.4.1.203 D86A site-directed mutagenesis Phaseolus lunatus
2.4.1.203 D87A site-directed mutagenesis Phaseolus lunatus
2.4.1.203 D87E site-directed mutagenesis Phaseolus lunatus
2.4.1.203 D87N site-directed mutagenesis Phaseolus lunatus
2.4.1.203 E85A site-directed mutagenesis Phaseolus lunatus
2.4.1.203 F149A site-directed mutagenesis Phaseolus lunatus
2.4.1.203 F149L site-directed mutagenesis Phaseolus lunatus
2.4.1.203 F149Y site-directed mutagenesis Phaseolus lunatus
2.4.1.203 L127A site-directed mutagenesis, no activity Phaseolus lunatus
2.4.1.203 L127F site-directed mutagenesis, no activity Phaseolus lunatus
2.4.1.203 L127M site-directed mutagenesis Phaseolus lunatus
2.4.1.203 L58A site-directed mutagenesis Phaseolus lunatus
2.4.1.203 Q55A site-directed mutagenesis Phaseolus lunatus
2.4.1.203 R54A site-directed mutagenesis Phaseolus lunatus
2.4.1.203 R59A site-directed mutagenesis, no activity Phaseolus lunatus
2.4.1.203 R59K site-directed mutagenesis Phaseolus lunatus
2.4.1.203 R59Q site-directed mutagenesis, no activity Phaseolus lunatus
2.4.1.203 T152F site-directed mutagenesis Phaseolus vulgaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.203 50000
-
 SDS-PAGE Phaseolus vulgaris
2.4.1.203 60000
-
 SDS-PAGE Phaseolus lunatus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.203 Glycine max Q8S3B9
-
-
2.4.1.203 Phaseolus lunatus Q8S3B6
-
-
2.4.1.203 Phaseolus vulgaris A7L745
-
-
2.4.1.215 Zea mays Q93XP7 phylogenic relationships between zeatin glycosyltransferases and closely related enzymes of bean, soybean, tomato, maize, rice, and Arabidopsis is studied
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.203 0.0000066
-
 T152F, no activity in wild type Phaseolus vulgaris
2.4.1.203 0.00001
-
 CTC151SFS mutant Phaseolus vulgaris
2.4.1.203 0.00001166
-
 F149L mutant Phaseolus lunatus
2.4.1.203 0.00001666
-
 F149Y mutant Phaseolus lunatus
2.4.1.203 0.0000216
-
 R59K mutant Phaseolus lunatus
2.4.1.203 0.0000233
-
 L127M mutant Phaseolus lunatus
2.4.1.203 0.0000316
-
 D87N mutant Phaseolus lunatus
2.4.1.203 0.0000533
-
 D87A mutant Phaseolus lunatus
2.4.1.203 0.0000916
-
 D87E mutant Phaseolus lunatus
2.4.1.203 0.000105
-
 L58A mutant Phaseolus lunatus
2.4.1.203 0.00015
-
 R54A mutant Phaseolus lunatus
2.4.1.203 0.000175
-
 D86A mutant Phaseolus lunatus
2.4.1.203 0.000186
-
 E85A mutant Phaseolus lunatus
2.4.1.203 0.0002
-
 wild type Phaseolus lunatus
2.4.1.203 0.000205
-
 Q55A mutant Phaseolus lunatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.203 UDP-glucose + trans-zeatin
-
 Phaseolus lunatus UDP + O-beta-D-glucosyl-trans-zeatin
-
 ?
2.4.1.203 UDP-glucose + trans-zeatin
-
 Phaseolus vulgaris UDP + O-beta-D-glucosyl-trans-zeatin
-
 ?
2.4.1.203 UDP-glucose + trans-zeatin
-
 Glycine max UDP + O-beta-D-glucosyl-trans-zeatin
-
 ?
2.4.1.215 UDP-glucose + cis-zeatin
-
 Zea mays UDP + O-beta-D-glucosyl-cis-zeatin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.203 zeatin glycosyltransferase GT Phaseolus lunatus
2.4.1.203 zeatin glycosyltransferase GT Phaseolus vulgaris
2.4.1.203 zeatin glycosyltransferase GT Glycine max
2.4.1.203 zeatin O-glucosyltransferase
-
 Phaseolus lunatus
2.4.1.203 zeatin O-glucosyltransferase
-
 Phaseolus vulgaris
2.4.1.203 zeatin O-glucosyltransferase
-
 Glycine max
2.4.1.203 ZOG1
-
 Phaseolus lunatus
2.4.1.203 ZOG1
-
 Phaseolus vulgaris
2.4.1.203 ZOG1
-
 Glycine max
2.4.1.215 zeatin glycosyltransferase GT Zea mays
2.4.1.215 zeatin O-glucosyltransferase
-
 Zea mays
2.4.1.215 ZOG1
-
 Zea mays