Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Caligiuri, A.; DArrigo, P.; Rosini, E.; Pedrocchi-Fantoni, G.; Tessaro, D.; Molla, G.; Servi, S.; Pollegioni, L.
    Activity of yeast D-amino acid oxidase on aromatic unnatural amino acids (2008), J. Mol. Catal. B, 50, 93-98.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.3 expressed in Escherichia coli BL21(DE3)pLysS cells Rhodotorula toruloides

Protein Variants

EC Number Protein Variants Comment Organism
1.4.3.3 M213G mutant shows lower activity and substrate specificity constant on the natural aromatic D-amino acids and higher acitivy on artificial amino acids compared to the wild type enzyme Rhodotorula toruloides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.3 0.01
-
 D-2-naphthyl-glycine wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C Rhodotorula toruloides
1.4.3.3 0.03
-
 D-1-naphthyl-alanine mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C Rhodotorula toruloides
1.4.3.3 0.03
-
 D-2-naphthyl-glycine mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C Rhodotorula toruloides
1.4.3.3 0.03
-
 D-4-chloro-phenylglycine wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C Rhodotorula toruloides
1.4.3.3 0.04
-
 D-2-naphthyl-alanine wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C Rhodotorula toruloides
1.4.3.3 0.04
-
 D-1-naphthyl-alanine wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C Rhodotorula toruloides
1.4.3.3 0.05
-
 D-1-naphthyl-glycine mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C Rhodotorula toruloides
1.4.3.3 0.06
-
 D-2-naphthyl-alanine mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C Rhodotorula toruloides
1.4.3.3 0.13
-
 D-2-chloro-phenylglycine wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C Rhodotorula toruloides
1.4.3.3 0.15
-
 D-homo-phenylalanine mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C Rhodotorula toruloides
1.4.3.3 0.2
-
 D-phenylalanine mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C Rhodotorula toruloides
1.4.3.3 0.2
-
 D-4-chloro-phenylglycine mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C Rhodotorula toruloides
1.4.3.3 0.3
-
 D-phenylalanine wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C Rhodotorula toruloides
1.4.3.3 0.3
-
 D-homo-phenylalanine wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C Rhodotorula toruloides
1.4.3.3 0.33
-
 D-1-naphthyl-glycine wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C Rhodotorula toruloides
1.4.3.3 0.4
-
 D-2-chloro-phenylglycine mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C Rhodotorula toruloides
1.4.3.3 0.5
-
 D-2-fluoro-phenylglycine mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C Rhodotorula toruloides
1.4.3.3 0.5
-
 D-2-fluoro-phenylglycine wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C Rhodotorula toruloides
1.4.3.3 0.6
-
 D-4-fluoro-phenylglycine mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C Rhodotorula toruloides
1.4.3.3 0.7
-
 D-4-fluoro-phenylglycine wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C Rhodotorula toruloides
1.4.3.3 1.6
-
 D-4-hydroxy-phenylglycine mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C Rhodotorula toruloides
1.4.3.3 2.1
-
 D-4-hydroxy-phenylglycine wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C Rhodotorula toruloides
1.4.3.3 3.5
-
 D-phenylglycine wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C Rhodotorula toruloides
1.4.3.3 5.2
-
 D-phenylglycine mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C Rhodotorula toruloides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.3 D-alanine + H2O + O2 Rhodotorula toruloides
-
 pyruvate + NH3 + H2O2
-
 ?
1.4.3.3 D-phenylalanine + H2O + O2 Rhodotorula toruloides
-
 phenylpyruvate + NH3 + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.3 Rhodotorula toruloides P80324
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.3 Hitrap chelating chromatography Rhodotorula toruloides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.3 D-1-naphthyl-alanine + H2O + O2
-
 Rhodotorula toruloides ?
-
 ?
1.4.3.3 D-1-naphthyl-glycine + H2O + O2
-
 Rhodotorula toruloides ?
-
 ?
1.4.3.3 D-2-chloro-phenylglycine + H2O + O2
-
 Rhodotorula toruloides (2-chlorophenyl)acetic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-2-fluoro-phenylglycine + H2O + O2
-
 Rhodotorula toruloides (2-fluorophenyl)acetic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-2-naphthyl-alanine + H2O + O2
-
 Rhodotorula toruloides ?
-
 ?
1.4.3.3 D-2-naphthyl-glycine + H2O + O2
-
 Rhodotorula toruloides ?
-
 ?
1.4.3.3 D-4-chloro-phenylglycine + H2O + O2
-
 Rhodotorula toruloides (4-chlorophenyl)acetic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-4-fluoro-phenylglycine + H2O + O2
-
 Rhodotorula toruloides (4-fluorophenyl)acetic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-4-hydroxy-phenylglycine + H2O + O2
-
 Rhodotorula toruloides (4-hydroxyphenyl)acetic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-alanine + H2O + O2
-
 Rhodotorula toruloides pyruvate + NH3 + H2O2
-
 ?
1.4.3.3 D-homo-phenylalanine + H2O + O2
-
 Rhodotorula toruloides ?
-
 ?
1.4.3.3 D-phenylalanine + H2O + O2
-
 Rhodotorula toruloides phenylpyruvate + NH3 + H2O2
-
 ?
1.4.3.3 D-phenylalanine + H2O + O2
-
 Rhodotorula toruloides ?
-
 ?
1.4.3.3 D-phenylglycine + H2O + O2
-
 Rhodotorula toruloides benzoylformic acid + NH3 + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.4.3.3 D-amino acid oxidase
-
 Rhodotorula toruloides
1.4.3.3 DAAO
-
 Rhodotorula toruloides

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.3 FAD FAD-dependent enzyme Rhodotorula toruloides