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Literature summary extracted from

  • Reddy, K.R.; Kayastha, A.M.
    Improved stability of urease upon coupling to alkylamine and arylamine glass and its analytical use (2006), J. Mol. Catal. B, 38, 104-112.
No PubMed abstract available

General Stability

EC Number General Stability Organism
3.5.1.5 the immobilized enzyme is more stable in wet condition than in semi-dry condition and storing it at 4°C is more favourable over 27°C. There is a tremendous decrease in activity when the immobilized urease is not stored in buffer (i.e. semi-dry condition) and storing it at 4°C improves the longevity. Both the alkyl and arylamine-urease in wet condition stored at 4°C are further reused (30times) and the residual activity are 40% and 20%, respectively Cajanus cajan

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.5 3.35
-
 Urea soluble enzyme Cajanus cajan
3.5.1.5 3.43
-
 Urea arylamine glass-immobilized urease Cajanus cajan
3.5.1.5 3.55
-
 Urea alkylamine glass-immobilized urease Cajanus cajan

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.5 Cajanus cajan
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.5
-
 Cajanus cajan

Storage Stability

EC Number Storage Stability Organism
3.5.1.5 4°C, 70 days, soluble enzyme loses 90% of its activity, alkylamine-immobilized enzyme loses 15% of the initial activity, the arylamine-immobilized enzyme loses 17% of its activity Cajanus cajan

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.5 urea + H2O
-
 Cajanus cajan CO2 + NH3
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.5 47
-
 soluble enzyme Cajanus cajan
3.5.1.5 77
-
 urease coupled to the alkylamine glass or arylamine glass Cajanus cajan

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.5 77
-
 15 min, soluble urease has a residual activity of 22%, alkylamine glass-immobilized enzyme and arylamine glass-immobilized urease retain 96% and 98%, respectively. Even after 90 min of incubation immobilized urease retains 50% of activity Cajanus cajan

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.5 6.8
-
 alkylamine glass-immobilized enzyme Cajanus cajan
3.5.1.5 7
-
 arylamine glass-immobilized enzyme Cajanus cajan
3.5.1.5 7.3
-
 soluble enzyme Cajanus cajan

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.5 3.5
-
 22°C, 12 h, soluble enzyme retains 5% of its original activity, alkylamine glass-immobilized enzyme and arylamine glass immobilized urease retain 37% and 38%, respectively Cajanus cajan