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Literature summary extracted from

  • Lejon, S.; Ellis, J.; Valegard, K.
    The last step in cephalosporin C formation revealed: crystal structures of deacetylcephalosporin C acetyltransferase from Acremonium chrysogenum in complexes with reaction intermediates (2008), J. Mol. Biol., 377, 935-944.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.1.175 biotechnology studies of the enzyme will be useful in gaining a full view of the possibilities for overcoming the acetyl transfer bottleneck and for the future development of cephalosporin antibiotics Acremonium chrysogenum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.175 into the pTWIN vector for expression in Escherichia coli BL21DE3 cells Acremonium chrysogenum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.175 different structures are solved, SeMet-DAC-AT with acetyl-CoA to a resolution of 2.4 A, DAC-AT with acetyl-CoA to 2.2 A, DAC-AT with DAC/cephalosporin C to 2.5 A, and DAC-AT with DAC to 2.6 A resolution Acremonium chrysogenum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.175 acetyl-CoA + deacetylcephalosporin C Acremonium chrysogenum
-
 CoA + cephalosporin C
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.175 Acremonium chrysogenum P39058
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.175 using a gravity-flow column containing chitin beads, cleavage of the chitin-binding domain and intein fusion partner is performed Acremonium chrysogenum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.175 acetyl-CoA + deacetylcephalosporin C
-
 Acremonium chrysogenum CoA + cephalosporin C
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.175 DAC-AT
-
 Acremonium chrysogenum
2.3.1.175 deacetylcephalosporin-C acetyltransferase
-
 Acremonium chrysogenum