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Literature summary extracted from

  • Li, Z.; Huang, Y.; Ge, J.; Fan, H.; Zhou, X.; Li, S.; Bartlam, M.; Wang, H.; Rao, Z.
    The crystal structure of MCAT from Mycobacterium tuberculosis reveals three new catalytic models (2007), J. Mol. Biol., 371, 1075-1083.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.39 expressed as a His-tagged fusion protein Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.39 crystal structure of MCAT is determined to 2.3 A Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.39 H90A Km only slightly increased compared to wild-type but Vmax strongly decreased compared to wild-type Mycobacterium tuberculosis
2.3.1.39 H90A/N155A lowest enzymatic activity Mycobacterium tuberculosis
2.3.1.39 N155A Km only slightly increased compared to wild-type but Vmax strongly decreased compared to wild-type Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.39 0.0167
-
 malonyl-CoA wild-type enzyme, Vmax (RFU/min): 123 Mycobacterium tuberculosis
2.3.1.39 0.0175
-
 malonyl-CoA mutant N155A, Vmax (RFU/min): 63.06 Mycobacterium tuberculosis
2.3.1.39 0.0191
-
 malonyl-CoA mutant H90A, Vmax (RFU/min): 39.65 Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.39 30000
-
-
 Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.39 Mycobacterium tuberculosis P9WNG5
-
-
2.3.1.39 Mycobacterium tuberculosis H37Rv P9WNG5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.39 using Ni-NTA chromatography, ion-exchange chromatography and gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.39 malonyl-CoA + acyl-carrier protein
-
 Mycobacterium tuberculosis CoA + malonyl-acyl-carrier protein
-
 ?
2.3.1.39 malonyl-CoA + acyl-carrier protein
-
 Mycobacterium tuberculosis H37Rv CoA + malonyl-acyl-carrier protein
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.39 monomer crystal structure Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.3.1.39 malonyl CoA-acyl carrier protein transacylase
-
 Mycobacterium tuberculosis
2.3.1.39 MCAT
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.39 37
-
 assay at Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.39 6.8
-
 assay at Mycobacterium tuberculosis