EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.219 | expression in Escherichia coli | Vitis vinifera |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.1.1.219 | purified recombinant enzyme in complex with NADPH and dihydroquercetin, X-ray diffraction structure determination and analysis at 1.8 A resolution | Vitis vinifera |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.219 | N133D | site-directed mutagenesis, | Vitis vinifera |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.219 | dihydrokaempferol + NADPH | Vitis vinifera | - |
cis-3,4-leucopelargonidin + NADP+ | - |
r | |
1.1.1.219 | dihydromyricetin + NADPH | Vitis vinifera | - |
leucodelphinidin + NADP+ | - |
r | |
1.1.1.219 | dihydroquercetin + NADPH | Vitis vinifera | - |
leucocyanidin + NADP+ | - |
r | |
1.1.1.219 | additional information | Vitis vinifera | the key enzyme in flavonoid biosynthesis catalyzes a late step in the biosynthesis of anthocyanins and condensed tannins, two flavonoid classes of importance to plant survival and human nutrition, overview | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.219 | Vitis vinifera | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.219 | recombinanet enzyme from Escherichia coli | Vitis vinifera |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.219 | dihydrokaempferol + NADPH | - |
Vitis vinifera | cis-3,4-leucopelargonidin + NADP+ | - |
r | |
1.1.1.219 | dihydromyricetin + NADPH | - |
Vitis vinifera | leucodelphinidin + NADP+ | - |
r | |
1.1.1.219 | dihydroquercetin + NADPH | - |
Vitis vinifera | leucocyanidin + NADP+ | - |
r | |
1.1.1.219 | additional information | the key enzyme in flavonoid biosynthesis catalyzes a late step in the biosynthesis of anthocyanins and condensed tannins, two flavonoid classes of importance to plant survival and human nutrition, overview | Vitis vinifera | ? | - |
? | |
1.1.1.219 | additional information | the specific residue at position 133, Asn or Asp, is involved in controling of the substrate recognition and recognition of the B-ring hydroxylation pattern of dihydroflavonols, structure-function relationship, overview | Vitis vinifera | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.219 | More | 3D structure of the ternary complex, overview | Vitis vinifera |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.219 | DFR | - |
Vitis vinifera |
1.1.1.219 | dihydroflavonol 4-reductase | - |
Vitis vinifera |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.219 | NADP+ | binding site structure, overview | Vitis vinifera | |
1.1.1.219 | NADPH | - |
Vitis vinifera |