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Literature summary extracted from

  • Arakawa, T.; Kawano, Y.; Kataoka, S.; Katayama, Y.; Kamiya, N.; Yohda, M.; Odaka, M.
    Structure of thiocyanate hydrolase: a new nitrile hydratase family protein with a novel five-coordinate cobalt(III) center (2007), J. Mol. Biol., 366, 1497-1509.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.5.8 hanging drop vapour diffusion method with 0.1 M potassium phosphate (pH 7.6) containing 1.5 M Na/K L-(+) tartrate or 0.1 M potassium phosphate (pH 7.2) containing 2 M ammonium sulfate for apo- or native SCNase, respectively Thiobacillus thioparus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.5.8 Co3+ contains a five-coordinate cobalt(III) center Thiobacillus thioparus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.5.8 240000
-
 gel filtration Thiobacillus thioparus

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.8 Thiobacillus thioparus
-
-
-
3.5.5.8 Thiobacillus thioparus THI115
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.5.8
-
 Thiobacillus thioparus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.5.8 thiocyanate + H2O
-
 Thiobacillus thioparus carbonyl sulfide + NH3 + OH-
-
 ?
3.5.5.8 thiocyanate + H2O
-
 Thiobacillus thioparus THI115 carbonyl sulfide + NH3 + OH-
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.5.8 heterododecamer x-ray crystallography Thiobacillus thioparus

Synonyms

EC Number Synonyms Comment Organism
3.5.5.8 SCNase
-
 Thiobacillus thioparus