EC Number | Application | Comment | Organism |
---|---|---|---|
2.4.1.1 | synthesis | maltodextrin phosphorylase from Pyrococcus furiosus is fused with the cellulose-binding domain of Clostridium cellulovorans serving as an aggregation module. After molecular cloning of the corresponding gene fusion construct and controlled expression in Escherichia coli BL21, 83% of total maltodextrin phosphorylase activity is displayed in active inclusion bodies. These active inclusion bodies are easily isolated by nonionic detergent treatment and directly used for maltodextrin conversion to alpha-D-glucose-1-phosphate in a repetitive batch mode. Only 10% of enzyme activity is lost after ten conversion cycles at optimum conditions | Pyrococcus furiosus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.1 | maltodextrin phosphorylase from Pyrococcus furiosus is fused with the cellulose-binding domain of Clostridium cellulovorans serving as an aggregation module. After molecular cloning of the corresponding gene fusion construct and controlled expression in Escherichia coli BL21, 83% of total maltodextrin phosphorylase activity is displayed in active inclusion bodies | Pyrococcus furiosus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.1 | Pyrococcus furiosus | - |
- |
- |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.1 | maltodextrin phosphorylase | - |
Pyrococcus furiosus |