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Literature summary extracted from

  • Kargiotidou, A.; Deli, D.; Galanopoulou, D.; Tsaftaris, A.; Farmaki, T.
    Low temperature and light regulate delta 12 fatty acid desaturases (FAD2) at a transcriptional level in cotton (Gossypium hirsutum) (2008), J. Exp. Bot., 59, 2043-2056.
    View publication on PubMedView publication on EuropePMC

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.6 Gossypium hirsutum Q8W2B9 cultivar Acala SJ2
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1.14.19.22 Gossypium hirsutum Q8W2B9 cultivar Acala SJ2
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Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.19.6 cotyledon very low expression level in roots Gossypium hirsutum
-
1.14.19.6 leaf very low expression level in new leaves Gossypium hirsutum
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1.14.19.6 root
-
 Gossypium hirsutum
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1.14.19.22 cotyledon
-
 Gossypium hirsutum
-
1.14.19.22 leaf
-
 Gossypium hirsutum
-
1.14.19.22 root
-
 Gossypium hirsutum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.6 oleic acid + reduced acceptor + O2
-
 Gossypium hirsutum linoleic acid + acceptor + H2O
-
 ?
1.14.19.22 1-acyl-2-oleoyl-sn-glycero-3-phosphocholine + ferrocytochrome b5 + O2 + H+
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 Gossypium hirsutum 1-acyl-2-linoleoyl-sn-glycero-3-phosphocholine + ferricytochrome b5 + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.19.6 delta 12 fatty acid desaturases
-
 Gossypium hirsutum
1.14.19.22 delta 12 fatty acid desaturase
-
 Gossypium hirsutum
1.14.19.22 FAD2-2 isozyme Gossypium hirsutum
1.14.19.22 FAD2-3 isozyme Gossypium hirsutum
1.14.19.22 FAD2-4 isozyme Gossypium hirsutum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.19.22 20 32 no difference in FAD2 expression is found in plants grown between 20°C and 32°C, isozymes FAD2-3 and FAD2-4 are induced under cold stress, FAD2-4 induction is significantly higher than FAD2-3, in contrast isozyme FAD2-2 is not affected by cold Gossypium hirsutum

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.22 NAD+
-
 Gossypium hirsutum

Expression

EC Number Organism Comment Expression
1.14.19.6 Gossypium hirsutum the induction of FAD2 is inhibited by incubation at 10°C under dark conditions but appears unaffected by light intensity down
1.14.19.6 Gossypium hirsutum induction of FAD2 expression following a 24 h incubation at 10°C is light dependent, no difference in FAD2 expression is found in plants grown between 32°C and 20°C additional information
1.14.19.6 Gossypium hirsutum mRNA accumulation shows a periodic behaviour, decreasing to the 22°C levels following a 48 h incubation and re-establishing ist 24 h induction after 96 h up