EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.1.99.29 | NH4+ | at 0.1-1 M in 20 mM sodium phosphate buffer pH 7.0, not due to increased ionic strenth | Leucoagaricus meleagris |
EC Number | Application | Comment | Organism |
---|---|---|---|
1.1.99.29 | degradation | lignocellulose degradation | Leucoagaricus meleagris |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.99.29 | alpha,alpha-dipyridyl | no activity | Leucoagaricus meleagris | |
1.1.99.29 | CN- | no activity | Leucoagaricus meleagris | |
1.1.99.29 | EDTA | no activity | Leucoagaricus meleagris | |
1.1.99.29 | F- | no activity | Leucoagaricus meleagris | |
1.1.99.29 | MoO42- | no activity | Leucoagaricus meleagris | |
1.1.99.29 | NaN3 | no activity | Leucoagaricus meleagris | |
1.1.99.29 | NH4+ | at 2 M in 20 mM sodium phosphate buffer pH 7.0 | Leucoagaricus meleagris | |
1.1.99.29 | p-hydroxymercuribenzoate | no activity | Leucoagaricus meleagris | |
1.1.99.29 | Phenanthroline | no activity | Leucoagaricus meleagris |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.99.29 | 0.13 | - |
Fe3+ | at 30°C with 50 mM glucose as susbtrate | Leucoagaricus meleagris | |
1.1.99.29 | 0.18 | - |
methyl-1,4-benzoquinone | at 30°C with 50 mM glucose as susbtrate | Leucoagaricus meleagris | |
1.1.99.29 | 0.22 | - |
3,5-di-tert-butyl-1,4-benzoquinone | at 30°C with 50 mM glucose as susbtrate | Leucoagaricus meleagris | |
1.1.99.29 | 0.54 | - |
L-arabinose | Fe3+ as electron acceptor | Leucoagaricus meleagris | |
1.1.99.29 | 0.55 | - |
2-chloro-1,4-benzoquinone | at 30°C with 50 mM glucose as susbtrate | Leucoagaricus meleagris | |
1.1.99.29 | 0.82 | - |
D-glucose | Fe3+ as electron acceptor | Leucoagaricus meleagris | |
1.1.99.29 | 0.92 | - |
2,5-chloro-1,4-benzoquinone | at 30°C with 50 mM glucose as susbtrate | Leucoagaricus meleagris | |
1.1.99.29 | 1.05 | - |
D-galactose | Fe3+ as electron acceptor | Leucoagaricus meleagris | |
1.1.99.29 | 1.82 | - |
1,4-benzoquinone | at 30°C with 50 mM glucose as susbtrate | Leucoagaricus meleagris | |
1.1.99.29 | 1.93 | - |
D-xylose | Fe3+ as electron acceptor | Leucoagaricus meleagris | |
1.1.99.29 | 6.82 | - |
cellobiose | Fe3+ as electron acceptor | Leucoagaricus meleagris | |
1.1.99.29 | 8.61 | - |
D-maltose | Fe3+ as electron acceptor | Leucoagaricus meleagris | |
1.1.99.29 | 10.8 | - |
D-xylobiose | Fe3+ as electron acceptor | Leucoagaricus meleagris | |
1.1.99.29 | 59.7 | - |
D-ribose | Fe3+ as electron acceptor | Leucoagaricus meleagris | |
1.1.99.29 | 79.1 | - |
D-talose | Fe3+ as electron acceptor | Leucoagaricus meleagris | |
1.1.99.29 | 108 | - |
D-mannose | Fe3+ as electron acceptor | Leucoagaricus meleagris | |
1.1.99.29 | 134 | - |
lactose | Fe3+ as electron acceptor | Leucoagaricus meleagris | |
1.1.99.29 | 137 | - |
L-sorbose | Fe3+ as electron acceptor | Leucoagaricus meleagris | |
1.1.99.29 | 153 | - |
D-fructose | Fe3+ as electron acceptor | Leucoagaricus meleagris | |
1.1.99.29 | 156 | - |
maltotriose | Fe3+ as electron acceptor | Leucoagaricus meleagris |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.99.29 | extracellular | - |
Leucoagaricus meleagris | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.99.29 | Ag+ | 37% inhibition of activity | Leucoagaricus meleagris | |
1.1.99.29 | Al3+ | no activity | Leucoagaricus meleagris | |
1.1.99.29 | Ca2+ | no activity | Leucoagaricus meleagris | |
1.1.99.29 | Cu2+ | no activity | Leucoagaricus meleagris | |
1.1.99.29 | Fe2+ | no activity | Leucoagaricus meleagris | |
1.1.99.29 | Fe3+ | 39% inhibition of activity | Leucoagaricus meleagris | |
1.1.99.29 | Hg2+ | 25% inhibition of activity | Leucoagaricus meleagris | |
1.1.99.29 | Mg2+ | no activity | Leucoagaricus meleagris | |
1.1.99.29 | Mn2+ | no activity | Leucoagaricus meleagris | |
1.1.99.29 | Ni2+ | no activity | Leucoagaricus meleagris | |
1.1.99.29 | Zn2+ | no activity | Leucoagaricus meleagris |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.99.29 | 46000 | - |
SDS-PAGE, PDHf, second band | Leucoagaricus meleagris |
1.1.99.29 | 66500 | - |
SDS-PAGE, gel filtration, MALDI MS | Leucoagaricus meleagris |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.99.29 | Leucoagaricus meleagris | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
1.1.99.29 | glycoprotein | carbohydrate content of 7% | Leucoagaricus meleagris |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.99.29 | using DEAE-Sephacel anion exchange chromatography and FPLC on phenyl-superose HR 10/10, Mono Q HR 5/5 and superose 12 HR 30/10 chromatography | Leucoagaricus meleagris |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|
EC Number | Storage Stability | Organism |
---|---|---|
1.1.99.29 | stable for 3 months at 4°C, in sodium phosphate buffer pH 7.0 | Leucoagaricus meleagris |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.99.29 | cellobiose + Fe3+ | - |
Leucoagaricus meleagris | ? | - |
? | |
1.1.99.29 | D-fructose + Fe3+ | - |
Leucoagaricus meleagris | ? | - |
? | |
1.1.99.29 | D-galactose + benzoquinone | - |
Leucoagaricus meleagris | 2-dehydro-D-galactose + hydroquinone | no 2,3-didehydro-d-galactose is detectable | ? | |
1.1.99.29 | D-galactose + Fe3+ | - |
Leucoagaricus meleagris | 2-dehydro-D-galactose + Fe2+ + H+ | - |
? | |
1.1.99.29 | D-glucose | pH 8.5 | Leucoagaricus meleagris | 3-dehydro-D-glucose + Fe2+ + H+ | - |
? | |
1.1.99.29 | D-glucose + 2,5-chloro-1,4-benzoquinone | pH 5 | Leucoagaricus meleagris | ? | - |
? | |
1.1.99.29 | D-glucose + 2,6-dichloroindophenol | pH 4 | Leucoagaricus meleagris | ? | - |
? | |
1.1.99.29 | D-glucose + 2-chloro-1,4-benzoquinone | pH 5 | Leucoagaricus meleagris | ? | - |
? | |
1.1.99.29 | D-glucose + 3,5-di-tert-butyl-1,4-benzoquinone | pH 6 | Leucoagaricus meleagris | ? | - |
? | |
1.1.99.29 | D-glucose + benzoquinone | pH 3 | Leucoagaricus meleagris | 2,3-didehydro-D-glucose + hydroquinone | - |
? | |
1.1.99.29 | D-glucose + methyl-1,4-benzoquinone | pH 8 | Leucoagaricus meleagris | ? | - |
? | |
1.1.99.29 | D-maltose + Fe3+ | - |
Leucoagaricus meleagris | ? | - |
? | |
1.1.99.29 | D-maltotriose + Fe3+ | - |
Leucoagaricus meleagris | ? | - |
? | |
1.1.99.29 | D-mannose + Fe3+ | - |
Leucoagaricus meleagris | ? | - |
? | |
1.1.99.29 | D-ribose + Fe3+ | - |
Leucoagaricus meleagris | ? | - |
? | |
1.1.99.29 | D-talose + Fe3+ | - |
Leucoagaricus meleagris | ? | - |
? | |
1.1.99.29 | D-xylobiose + ferricenium ion | - |
Leucoagaricus meleagris | ? | - |
r | |
1.1.99.29 | D-xylose + Fe3+ | - |
Leucoagaricus meleagris | ? | - |
? | |
1.1.99.29 | L-arabinose + Fe3+ | - |
Leucoagaricus meleagris | ? | - |
? | |
1.1.99.29 | L-sorbose + Fe3+ | - |
Leucoagaricus meleagris | ? | - |
? | |
1.1.99.29 | L-xylobiose + Fe3+ | - |
Leucoagaricus meleagris | ? | - |
? | |
1.1.99.29 | lactose + Fe3+ | - |
Leucoagaricus meleagris | ? | - |
? | |
1.1.99.29 | maltotriose + ferricenium ion | - |
Leucoagaricus meleagris | ? | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.99.29 | monomer | gel filtration | Leucoagaricus meleagris |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.99.29 | PDH | - |
Leucoagaricus meleagris |
1.1.99.29 | pyranose dehydrogenase | - |
Leucoagaricus meleagris |
1.1.99.29 | pyranose:acceptor oxidoreductase | - |
Leucoagaricus meleagris |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.99.29 | 63 | - |
- |
Leucoagaricus meleagris |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.99.29 | 50 | - |
50% activity loss after incubation at 50°C for 50 h | Leucoagaricus meleagris |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.99.29 | 2 | - |
for glucose and 1,4-benzoquinone as substrates | Leucoagaricus meleagris |
1.1.99.29 | 9 | - |
Fe3+ as electron acceptor, with bisTris propane buffer | Leucoagaricus meleagris |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.99.29 | 2 | 10 | depending on the electron acceptor and with d-glucose as electron donor, has 2 pH optima for some substrates | Leucoagaricus meleagris |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.1.99.29 | 7 | - |
80% maximum stability at pH 7 | Leucoagaricus meleagris |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.99.29 | FAD | 5 FAD covalently bound | Leucoagaricus meleagris |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.1.99.29 | Leucoagaricus meleagris | isoelectric focusing, 4 isoforms | - |
4.2 |
1.1.99.29 | Leucoagaricus meleagris | isoelectric focusing, 4 isoforms | - |
4.25 |
1.1.99.29 | Leucoagaricus meleagris | isoelectric focusing, 4 isoforms | - |
4.35 |
1.1.99.29 | Leucoagaricus meleagris | isoelectric focusing, 4 isoforms | - |
4.45 |